Overview
- External ontology
- mediadive.medium:J465 (source link unavailable) — CHOPPED MEAT MEDIUM WITH CARBOHYDRATES
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=465
Composition
flowchart LR
CultureMech_002813["chopped_meat_medium_with_carbohydrates"]
ing0_Ground_beef["Ground beef"]
CultureMech_002813 --> ing0_Ground_beef
ing1_NaOH["NaOH"]
CultureMech_002813 --> ing1_NaOH
CHEBI_32145["CHEBI:32145"]
ing1_NaOH -.-> CHEBI_32145
ing2_Casitone["Casitone"]
CultureMech_002813 --> ing2_Casitone
ing3_Yeast_extract["Yeast extract"]
CultureMech_002813 --> ing3_Yeast_extract
ing4_K2HPO4["K2HPO4"]
CultureMech_002813 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Resazurin["Resazurin"]
CultureMech_002813 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Ground beef | 487.805 G_PER_L | Copied from referenced JCM Medium 461 | |
| 2 |
NaOH
HO.Na 39.997 g/mol
|
25 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Copied from referenced JCM Medium 461 |
| 3 | Casitone | 29.2683 G_PER_L | Copied from referenced JCM Medium 461 | |
| 4 | Yeast extract | 4.87805 G_PER_L | Copied from referenced JCM Medium 461 | |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
4.87805 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Copied from referenced JCM Medium 461 |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00097561 G_PER_L |
CHEBI:8806
Resazurin
|
Copied from referenced JCM Medium 461 |
Preparation
- Use Medium No. 461 with 0.4% glucose, 0.1% cellobiose, 0.1% maltose and 0.1% soluble starch.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.682759Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J465 |
| 2026-02-03T11:26:55.003015 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:15.881015Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002813 |
| 2026-03-17T19:45:29.336962+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CHOPPED MEAT MEDIUM WITH CARBOHYDRATES" to "chopped_meat_medium_with_carbohydrates" for consistent matching |
| 2026-04-04T18:49:19.700673 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from chopped_meat_medium (CultureMech:002810) |
| 2026-05-16T08:57:04.612346+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:40:17.302505+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.982177+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |