bacillus_okhensis_medium

CultureMech:002825 bacterial COMPLEX LIQUID
Original name: BACILLUS OKHENSIS MEDIUM

Overview

External ontology
mediadive.medium:J476 (source link unavailable) — BACILLUS OKHENSIS MEDIUM
Applications
  • Microbial cultivation
pH
9.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=476

Composition

flowchart LR
    CultureMech_002825["bacillus_okhensis_medium"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_002825 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002825 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Peptone["Peptone"]
    CultureMech_002825 --> ing2_Peptone
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002825 --> ing3_Yeast_extract
    ing4_NaCl["NaCl"]
    CultureMech_002825 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17634
D-glucose
2 KH2PO4
H2O4P.K 136.084 g/mol
10 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Peptone 5 G_PER_L
4 Yeast extract 5 G_PER_L
5 NaCl
Cl.Na 58.443 g/mol
50 G_PER_L CHEBI:26710
sodium chloride

Preparation

  1. Mix components and autoclave. After cooling down to room temperature, adjust pH to 9.0 with 20% (w/v) Na2CO3 (sterilized by autoclaving).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.702262Z mediadive-import Imported from MediaDive Source: JCM, ID: J476
2026-03-13T06:19:15.926137Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002825
2026-03-17T06:29:51.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.388243+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BACILLUS OKHENSIS MEDIUM" to "bacillus_okhensis_medium" for consistent matching
2026-06-05T07:40:50.304125+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.730544+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:33:27.670564+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.