Overview
- External ontology
- mediadive.medium:J478 (source link unavailable) — MODIFIED MMJHS MEDIUM FOR GO25
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=478
Composition
flowchart LR
CultureMech_002827["modified_mmjhs_medium_for_go25"]
ing0_NaCl["NaCl"]
CultureMech_002827 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_K2HPO4["K2HPO4"]
CultureMech_002827 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_KH2PO4["KH2PO4"]
CultureMech_002827 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002827 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_NH4Cl["NH4Cl"]
CultureMech_002827 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_NaNO3["NaNO3"]
CultureMech_002827 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002827 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002827 --> ing7_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_002827 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002827 --> ing9_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing9_NiCl2_x_6_H2O -.-> CHEBI_34887
ing10_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002827 --> ing10_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing10_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing11_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
CultureMech_002827 --> ing11_Fe2_SO4_3_x_n_H2O
CHEBI_53438["CHEBI:53438"]
ing11_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
ing12_H2WO4["H2WO4"]
CultureMech_002827 --> ing12_H2WO4
CHEBI_36272["CHEBI:36272"]
ing12_H2WO4 -.-> CHEBI_36272
ing13_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002827 --> ing13_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing13_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing14_NaHCO3["NaHCO3"]
CultureMech_002827 --> ing14_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing14_NaHCO3 -.-> CHEBI_32139
ing15_Sulfur["Sulfur"]
CultureMech_002827 --> ing15_Sulfur
CHEBI_26833["CHEBI:26833"]
ing15_Sulfur -.-> CHEBI_26833
ing16_Resazurin["Resazurin"]
CultureMech_002827 --> ing16_Resazurin
CHEBI_8806["CHEBI:8806"]
ing16_Resazurin -.-> CHEBI_8806
ing17_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002827 --> ing17_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing17_Nitrilotriacetic_acid -.-> CHEBI_44557
ing18_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002827 --> ing18_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing18_MnSO4_x_n_H2O -.-> CHEBI_86360
ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002827 --> ing19_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002827 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002827 --> ing21_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
ing22_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002827 --> ing22_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing22_AlK_SO4_2 -.-> CHEBI_86463
ing23_H3BO3["H3BO3"]
CultureMech_002827 --> ing23_H3BO3
CHEBI_33118["CHEBI:33118"]
ing23_H3BO3 -.-> CHEBI_33118
ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002827 --> ing24_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing25_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_002827 --> ing25_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing25_SrCl2_x_6_H2O -.-> CHEBI_36385
ing26_NaBr["NaBr"]
CultureMech_002827 --> ing26_NaBr
CHEBI_63004["CHEBI:63004"]
ing26_NaBr -.-> CHEBI_63004
ing27_KI["KI"]
CultureMech_002827 --> ing27_KI
CHEBI_8346["CHEBI:8346"]
ing27_KI -.-> CHEBI_8346
ing28_Biotin["Biotin"]
CultureMech_002827 --> ing28_Biotin
CHEBI_15956["CHEBI:15956"]
ing28_Biotin -.-> CHEBI_15956
ing29_Folic_acid["Folic acid"]
CultureMech_002827 --> ing29_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing29_Folic_acid -.-> CHEBI_27470
CultureMech_002827 --> more["...8 more"]
Ingredients (38)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
19.7824 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.890208 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.692384 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.791296 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.24728 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
0.98912 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.95648 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.96736 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.326409 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 10 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.00098912 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 11 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.00098912 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 12 |
Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
|
0.0098912 G_PER_L |
CHEBI:53438
iron(3+) sulfate
|
|
| 13 |
H2WO4
H2O4W 249.852 g/mol
|
0.00098912 G_PER_L |
CHEBI:36272
tungstic acid
|
|
| 14 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1.97824 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 15 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.97824 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 16 |
Sulfur
S 32.067 g/mol
|
9.8912 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 17 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00098912 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 18 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 19 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 20 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.5 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 22 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 23 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.02 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 24 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 25 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 26 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.01 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 27 |
NaBr
Br.Na 102.894 g/mol
|
0.01 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 28 |
KI
I.K 166.002 g/mol
|
0.01 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 29 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 30 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 31 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 32 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 33 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 34 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 35 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 36 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 37 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 38 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix ingredients except Trace vitamins, NaHCO3 and sulfur, adjust pH to 6.8 and autoclave. Filter-sterilize Trace vitamins and 5% NaHCO3 solution and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing appropriate amount of sulfur (e.g., 20 ml of medium in 120 ml serum bottles containing 0.2 g sulfur) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Pressurize the inoculated bottle to 200 kPa H2-CO2 (4:1, v/v).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.705405Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J478 |
| 2026-03-13T06:19:15.940609Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002827 |
| 2026-03-17T06:30:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.404120+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED MMJHS MEDIUM FOR GO25" to "modified_mmjhs_medium_for_go25" for consistent matching |
| 2026-06-05T07:41:37.361515+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 35 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.818097+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |