reinforced_clostridial_medium_with_4_sea_salt

CultureMech:002832 bacterial COMPLEX SOLID_AGAR
Original name: REINFORCED CLOSTRIDIAL MEDIUM WITH 4% SEA SALT

Overview

External ontology
mediadive.medium:J482 (source link unavailable) — REINFORCED CLOSTRIDIAL MEDIUM WITH 4% SEA SALT
Applications
  • Microbial cultivation
Notes
JCM Medium 482 records JCM Medium 482 reinforced clostridial medium with 4% sea salt with 38 g/L Reinforced clostridial medium (BD-Difco), 40.0 g/L Sea salts (Sigma), 15 g/L Agar, 1 L distilled water, and the JCM default autoclaving at 121 C for 15 min. The commercial BD-Difco base and Sigma sea-salt mixture are retained as opaque unmapped components because JCM does not disclose their subcomposition.

Composition

flowchart LR
    CultureMech_002832["reinforced_clostridial_medium_with_4_sea_salt"]
    ing0_Reinforced_clostridial_medium__BD_Difco_["Reinforced clostridial medium (BD-Difco)"]
    CultureMech_002832 --> ing0_Reinforced_clostridial_medium__BD_Difco_
    ing1_Sea_salts__Sigma_["Sea salts (Sigma)"]
    CultureMech_002832 --> ing1_Sea_salts__Sigma_
    ing2_Agar["Agar"]
    CultureMech_002832 --> ing2_Agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar -.-> CHEBI_2509
    ing3_Distilled_water["Distilled water"]
    CultureMech_002832 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Reinforced clostridial medium (BD-Difco) 38.0 G_PER_L JCM Medium 482 lists 38 g/L commercial BD-Difco Reinforced Clostridial Medium but does not disclose its component recipe.
2 Sea salts (Sigma) 40.0 G_PER_L JCM Medium 482 lists 40.0 g/L commercial Sigma sea salts; this heterogeneous salt mixture is retained as an opaque source-qualified component.
3 Agar 15.0 G_PER_L CHEBI:2509
agar
 JCM Medium 482 lists 15.0 g/L Agar.
4 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 JCM Medium 482 lists 1.0 L Distilled water.

Preparation

  1. Autoclave at 121 C for 15 min.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.717707Z mediadive-import Imported from MediaDive Source: JCM, ID: J482
2026-03-13T06:19:15.967930Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002832
2026-03-17T06:29:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.436908+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "REINFORCED CLOSTRIDIAL MEDIUM WITH 4% SEA SALT" to "reinforced_clostridial_medium_with_4_sea_salt" for consistent matching
2026-06-05T07:41:44.161339+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_jcm_j481_j482_sea_salt_score15.py RESOLVED_JCM_481_482_SEA_SALT_SCORE15 Corrected the JCM 481/482 reinforced-clostridial sea-salt formula, grounded water and agar, added JCM default autoclaving, and marked the BD-Difco base and Sigma sea salts as intentionally opaque commercial ingredients.