catonella_ureilytica_medium

Overview

External ontology

mediadive.medium:J489 (source link unavailable) — CATONELLA UREILYTICA MEDIUM

Applications

• Microbial cultivation

pH

7.4

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=489

Composition

flowchart LR
    CultureMech_002839["catonella_ureilytica_medium"]
    ing0_Casitone["Casitone"]
    CultureMech_002839 --> ing0_Casitone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002839 --> ing1_Yeast_extract
    ing2_Na2HPO4["Na2HPO4"]
    CultureMech_002839 --> ing2_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4 -.-> CHEBI_34683
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002839 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_Urea["Urea"]
    CultureMech_002839 --> ing4_Urea
    CHEBI_16199["CHEBI:16199"]
    ing4_Urea -.-> CHEBI_16199
    ing5_Sodium_thioglycolate["Sodium thioglycolate"]
    CultureMech_002839 --> ing5_Sodium_thioglycolate
    CHEBI_86481["CHEBI:86481"]
    ing5_Sodium_thioglycolate -.-> CHEBI_86481
    ing6_Resazurin["Resazurin"]
    CultureMech_002839 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806

Ingredients (7)

Preparation

1. Mix components except urea and sodium thioglycolate, bring to a boil for a few seconds and cool to room temperature under a N2-CO2 (4:1, v/v) gas mixture. Add sodium thioglycolate, dispense the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add 10% urea solution (filter-sterilized and stored under a N2 gas atmosphere) to the medium and adjust pH to 7.4.

Target organisms

No target organisms are recorded.

Provenance