Overview
- External ontology
- mediadive.medium:J494 (source link unavailable) — MMJ-MH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=494
Composition
flowchart LR
CultureMech_002845["mmj_mh_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002845 --> ing0_Yeast_extract
ing1_Tryptone["Tryptone"]
CultureMech_002845 --> ing1_Tryptone
ing2_Sulfur["Sulfur"]
CultureMech_002845 --> ing2_Sulfur
CHEBI_26833["CHEBI:26833"]
ing2_Sulfur -.-> CHEBI_26833
ing3_Resazurin["Resazurin"]
CultureMech_002845 --> ing3_Resazurin
CHEBI_8806["CHEBI:8806"]
ing3_Resazurin -.-> CHEBI_8806
ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002845 --> ing4_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_x_9_H2O -.-> CHEBI_76209
ing5_NaCl["NaCl"]
CultureMech_002845 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_K2HPO4["K2HPO4"]
CultureMech_002845 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002845 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_NH4Cl["NH4Cl"]
CultureMech_002845 --> ing8_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing8_NH4Cl -.-> CHEBI_31206
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002845 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002845 --> ing10_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing10_MgCl2_x_6_H2O -.-> CHEBI_86345
ing11_KCl["KCl"]
CultureMech_002845 --> ing11_KCl
CHEBI_32588["CHEBI:32588"]
ing11_KCl -.-> CHEBI_32588
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002845 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002845 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002845 --> ing14_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing14_Na2SeO3_x_5_H2O -.-> CHEBI_131361
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | ||
| 2 | Tryptone | 1 G_PER_L | ||
| 3 |
Sulfur
S 32.067 g/mol
|
3 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 4 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 5 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.18 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 12 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.001 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 15 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.001 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Mix ingredients except sulfur and Na2S x 9H2O, adjust pH to 5.0 and autoclave under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a stream of a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add 5% Na2S x 9H2O (autoclaved and stocked under a N2 atmosphere) to the medium. Readjust pH to 5.0 - 5.3. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.748987Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J494 |
| 2026-03-13T06:19:16.047510Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002845 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.534484+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJ-MH MEDIUM" to "mmj_mh_medium" for consistent matching |
| 2026-06-05T07:41:35.498006+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.289752+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |