mmj_do_medium

CultureMech:002846 bacterial COMPLEX LIQUID
Original name: MMJ-DO MEDIUM

Overview

External ontology
mediadive.medium:J495 (source link unavailable) — MMJ-DO MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=495

Composition

flowchart LR
    CultureMech_002846["mmj_do_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002846 --> ing0_Yeast_extract
    ing1_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002846 --> ing1_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing1_Sodium_pyruvate -.-> CHEBI_50144
    ing2_Sodium_lactate["Sodium lactate"]
    CultureMech_002846 --> ing2_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing2_Sodium_lactate -.-> CHEBI_75228
    ing3_Sodium_ascorbate["Sodium ascorbate"]
    CultureMech_002846 --> ing3_Sodium_ascorbate
    CHEBI_113451["CHEBI:113451"]
    ing3_Sodium_ascorbate -.-> CHEBI_113451
    ing4_NaHCO3["NaHCO3"]
    CultureMech_002846 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Resazurin["Resazurin"]
    CultureMech_002846 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_NaCl["NaCl"]
    CultureMech_002846 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_K2HPO4["K2HPO4"]
    CultureMech_002846 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002846 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_NH4Cl["NH4Cl"]
    CultureMech_002846 --> ing9_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing9_NH4Cl -.-> CHEBI_31206
    ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002846 --> ing10_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing11_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002846 --> ing11_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing11_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing12_KCl["KCl"]
    CultureMech_002846 --> ing12_KCl
    CHEBI_32588["CHEBI:32588"]
    ing12_KCl -.-> CHEBI_32588
    ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002846 --> ing13_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002846 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002846 --> ing15_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing15_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.1 G_PER_L
2 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.5 G_PER_L CHEBI:50144
sodium pyruvate
3 Sodium lactate
C3H5O3.Na 112.06 g/mol
0.5 G_PER_L CHEBI:75228
sodium lactate
4 Sodium ascorbate
C6H7O6.Na 198.106 g/mol
0.1 G_PER_L CHEBI:113451
sodium ascorbate
5 NaHCO3
CHO3.Na 84.006 g/mol
1 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin
7 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
8 K2HPO4
HO4P.2K 174.174 g/mol
0.14 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.5 G_PER_L CHEBI:86158
calcium chloride dihydrate
10 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
11 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
12 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.18 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
13 KCl
Cl.K 74.551 g/mol
0.33 G_PER_L CHEBI:32588
potassium chloride
14 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.01 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.001 G_PER_L CHEBI:34887
nickel dichloride
16 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.001 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Mix ingredients except NaHCO3, adjust pH to 7.0 with NaOH and autoclave under a H2-CO2 (4:1, v/v) gas mixture. Filter-sterilize 8% NaHCO3 solution and add to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.751812Z mediadive-import Imported from MediaDive Source: JCM, ID: J495
2026-03-13T06:19:16.055009Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002846
2026-03-17T06:30:11.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.543758+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MMJ-DO MEDIUM" to "mmj_do_medium" for consistent matching
2026-06-05T07:41:35.441642+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.269514+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.