modified_biebl_and_pfennigs_medium

CultureMech:002847 bacterial COMPLEX LIQUID
Original name: MODIFIED BIEBL AND PFENNIG'S MEDIUM

Overview

External ontology
mediadive.medium:J496 (source link unavailable) — MODIFIED BIEBL AND PFENNIG'S MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=496

Composition

flowchart LR
    CultureMech_002847["modified_biebl_and_pfennigs_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002847 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002847 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002847 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002847 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_002847 --> ing4_Yeast_extract
    ing5_NaCl["NaCl"]
    CultureMech_002847 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Sorbitol["Sorbitol"]
    CultureMech_002847 --> ing6_Sorbitol
    CHEBI_30911["CHEBI:30911"]
    ing6_Sorbitol -.-> CHEBI_30911
    ing7_Na2_EDTA["Na2-EDTA"]
    CultureMech_002847 --> ing7_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing7_Na2_EDTA -.-> CHEBI_64734
    ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002847 --> ing8_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002847 --> ing9_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002847 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_ZnCl2["ZnCl2"]
    CultureMech_002847 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002847 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002847 --> ing13_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing14_H3BO3["H3BO3"]
    CultureMech_002847 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002847 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.499501 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.24975 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.999001 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.599401 G_PER_L CHEBI:31206
ammonium chloride
5 Yeast extract 0.2997 G_PER_L
6 NaCl
Cl.Na 58.443 g/mol
19.98 G_PER_L CHEBI:26710
sodium chloride
7 Sorbitol
C6H14O6 182.172 g/mol
2.997 G_PER_L CHEBI:30911
glucitol
8 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
3 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
9 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
10 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.05 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.042 G_PER_L CHEBI:49976
zinc dichloride
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
14 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.018 G_PER_L CHEBI:75213
sodium molybdate dihydrate
15 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate

Preparation

  1. Add the following component after autoclaving:
  2. Autoclave separately.
  3. Adjust pH to 6.8.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.754602Z mediadive-import Imported from MediaDive Source: JCM, ID: J496
2026-03-13T06:19:16.062692Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002847
2026-03-17T06:30:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.553331+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED BIEBL AND PFENNIG'S MEDIUM" to "modified_biebl_and_pfennigs_medium" for consistent matching
2026-06-05T07:40:17.376482+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.000649+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.