Overview
- External ontology
- mediadive.medium:J506 (source link unavailable) — IGNISPHAERA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=506
Composition
flowchart LR
CultureMech_002856["ignisphaera_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002856 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KH2PO4["KH2PO4"]
CultureMech_002856 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002856 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002856 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Yeast_extract["Yeast extract"]
CultureMech_002856 --> ing4_Yeast_extract
ing5_Trypticase_peptone["Trypticase peptone"]
CultureMech_002856 --> ing5_Trypticase_peptone
ing6_Starch["Starch"]
CultureMech_002856 --> ing6_Starch
CHEBI_28017["CHEBI:28017"]
ing6_Starch -.-> CHEBI_28017
ing7_L_Cystine["L-Cystine"]
CultureMech_002856 --> ing7_L_Cystine
CHEBI_16283["CHEBI:16283"]
ing7_L_Cystine -.-> CHEBI_16283
ing8_Resazurin["Resazurin"]
CultureMech_002856 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002856 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002856 --> ing10_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing10_MnSO4_x_n_H2O -.-> CHEBI_86360
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002856 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_H3BO3["H3BO3"]
CultureMech_002856 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002856 --> ing13_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002856 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002856 --> ing15_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
ing16_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002856 --> ing16_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing16_FeCl3_x_6_H2O -.-> CHEBI_86254
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.28968 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.277778 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.277778 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0734127 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 | Yeast extract | 0.0992064 G_PER_L | ||
| 6 | Trypticase peptone | 1.98413 G_PER_L | ||
| 7 | Starch | 1.98413 G_PER_L |
CHEBI:28017
starch
|
|
| 8 |
L-Cystine
C6H12N2O4S2 240.306 g/mol
|
0.297619 G_PER_L |
CHEBI:16283
L-cystine
|
|
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000992064 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
6 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.0022 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0005 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.0005 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
1.6e-05 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
2.5e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
4.6e-05 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 17 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.28 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Preparation
- Mix components thoroughly, adjust pH to 6.5 and autoclave under a N2 atmosphere. After cooling, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
- Dissolve at 1,000-fold concentration in acidified water (0.5 ml concentrated H2SO4 per liter), then dilute.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.783759Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J506 |
| 2026-03-13T06:19:16.112502Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002856 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.612623+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "IGNISPHAERA MEDIUM" to "ignisphaera_medium" for consistent matching |
| 2026-06-05T07:40:17.386745+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.004429+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |