marichromatium_imhoffii_medium (CultureMech:002868)

Overview

External ontology

mediadive.medium:J517 (source link unavailable) — MARICHROMATIUM IMHOFFII MEDIUM

Applications

Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=517

Composition

flowchart LR
    CultureMech_002868["marichromatium_imhoffii_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002868 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002868 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_NaCl["NaCl"]
    CultureMech_002868 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002868 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002868 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Sorbitol["Sorbitol"]
    CultureMech_002868 --> ing5_Sorbitol
    CHEBI_30911["CHEBI:30911"]
    ing5_Sorbitol -.-> CHEBI_30911
    ing6_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002868 --> ing6_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Sodium_pyruvate -.-> CHEBI_50144
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002868 --> ing7_Yeast_extract
    ing8_Ferric_citrate["Ferric citrate"]
    CultureMech_002868 --> ing8_Ferric_citrate
    CHEBI_144421["CHEBI:144421"]
    ing8_Ferric_citrate -.-> CHEBI_144421
    ing9_HCl["HCl"]
    CultureMech_002868 --> ing9_HCl
    CHEBI_17883["CHEBI:17883"]
    ing9_HCl -.-> CHEBI_17883
    ing10_ZnCl2["ZnCl2"]
    CultureMech_002868 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002868 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002868 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002868 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002868 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002868 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002868 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (17)

#	Ingredient	Concentration	Ontology	Notes
1	KH2PO4 H2O4P.K 136.084 g/mol	0.497018 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Copied from referenced JCM Medium 516
2	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.994036 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Copied from referenced JCM Medium 516
3	NaCl Cl.Na 58.443 g/mol	19.8807 G_PER_L	CHEBI:26710 sodium chloride	Copied from referenced JCM Medium 516
4	NH4Cl Cl.H4N 53.492 g/mol	0.596421 G_PER_L	CHEBI:31206 ammonium chloride	Copied from referenced JCM Medium 516
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.149105 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Copied from referenced JCM Medium 516
6	Sorbitol C6H14O6 182.172 g/mol	2.98211 G_PER_L	CHEBI:30911 glucitol	Copied from referenced JCM Medium 516
7	Sodium pyruvate C3H3O3.Na 110.044 g/mol	2.98211 G_PER_L	CHEBI:50144 sodium pyruvate	Copied from referenced JCM Medium 516
8	Yeast extract	0.397614 G_PER_L		Copied from referenced JCM Medium 516
9	Ferric citrate C6H5FeO7 244.944 g/mol	5 G_PER_L	CHEBI:144421 iron(III) citrate	Copied from referenced JCM Medium 516
10	HCl HCl 36.461 g/mol	1 G_PER_L	CHEBI:17883 hydrogen chloride	Copied from referenced JCM Medium 516
11	ZnCl2 2Cl.Zn 136.296 g/mol	0.0699301 G_PER_L	CHEBI:49976 zinc dichloride	Copied from referenced JCM Medium 516
12	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0999001 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Copied from referenced JCM Medium 516
13	H3BO3 H3BO3 61.833 g/mol	0.0599401 G_PER_L	CHEBI:33118 boric acid	Copied from referenced JCM Medium 516
14	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.1998 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Copied from referenced JCM Medium 516
15	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.01998 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Copied from referenced JCM Medium 516
16	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.01998 G_PER_L	CHEBI:34887 nickel dichloride	Copied from referenced JCM Medium 516
17	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.03996 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Copied from referenced JCM Medium 516

Preparation

Use Rhodobium gokurnum medium (see Medium No. 516) with 0.5 to 1.0 mM Na2S.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:16.818696Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J517
2026-02-03T11:26:55.175261	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:19:16.209120Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002868
2026-03-17T19:45:29.724709+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MARICHROMATIUM IMHOFFII MEDIUM" to "marichromatium_imhoffii_medium" for consistent matching
2026-04-04T21:48:41.537667	resolve_option_c_references	RESOLVED_REFERENCE	Resolved reference by copying from rhodobium_gokurnum_medium (CultureMech:002867)
2026-04-04T22:07:25.089144	resolve_option_c_references	RESOLVED_REFERENCE	Resolved reference by copying from rhodobium_gokurnum_medium (CultureMech:002867)
2026-05-16T08:57:38.016161+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(2 keys) -> list(1 entries)
2026-06-05T07:41:12.718582+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.844525+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.