Overview
- External ontology
- mediadive.medium:J524 (source link unavailable) — METHANE OXIDIZING BACTERIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=524
Composition
flowchart LR
CultureMech_002875["methane_oxidizing_bacterium_medium"]
ing0_NaCl["NaCl"]
CultureMech_002875 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KH2PO4["KH2PO4"]
CultureMech_002875 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002875 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002875 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002875 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_002875 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_NaNO3["NaNO3"]
CultureMech_002875 --> ing6_NaNO3
CHEBI_63005["CHEBI:63005"]
ing6_NaNO3 -.-> CHEBI_63005
ing7_NH4Cl["NH4Cl"]
CultureMech_002875 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
CultureMech_002875 --> ing8_Na2SiO3_x_9_H2O
CHEBI_132108["CHEBI:132108"]
ing8_Na2SiO3_x_9_H2O -.-> CHEBI_132108
ing9_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
CultureMech_002875 --> ing9_Fe2_SO4_3_x_n_H2O
CHEBI_53438["CHEBI:53438"]
ing9_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
ing10_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002875 --> ing10_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing10_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002875 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_NaHCO3["NaHCO3"]
CultureMech_002875 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
ing13_CuSO4["CuSO4"]
CultureMech_002875 --> ing13_CuSO4
CHEBI_23414["CHEBI:23414"]
ing13_CuSO4 -.-> CHEBI_23414
ing14_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002875 --> ing14_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing14_Nitrilotriacetic_acid -.-> CHEBI_44557
ing15_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002875 --> ing15_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing15_MnSO4_x_n_H2O -.-> CHEBI_86360
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002875 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002875 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002875 --> ing18_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
ing19_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002875 --> ing19_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing19_AlK_SO4_2 -.-> CHEBI_86463
ing20_H3BO3["H3BO3"]
CultureMech_002875 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002875 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_Biotin["Biotin"]
CultureMech_002875 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Folic_acid["Folic acid"]
CultureMech_002875 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002875 --> ing24_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing24_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing25_Thiamine_HCl["Thiamine HCl"]
CultureMech_002875 --> ing25_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing25_Thiamine_HCl -.-> CHEBI_49105
ing26_Riboflavin["Riboflavin"]
CultureMech_002875 --> ing26_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing26_Riboflavin -.-> CHEBI_17015
ing27_Nicotinic_acid["Nicotinic acid"]
CultureMech_002875 --> ing27_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing27_Nicotinic_acid -.-> CHEBI_15940
ing28_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002875 --> ing28_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing28_Calcium_pantothenate -.-> CHEBI_31345
ing29_Vitamin_B12["Vitamin B12"]
CultureMech_002875 --> ing29_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing29_Vitamin_B12 -.-> CHEBI_176843
CultureMech_002875 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
29.5276 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.137795 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.787402 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.34646 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.13386 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.324803 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
NaNO3
NO3.Na 84.994 g/mol
|
0.246063 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.246063 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
|
0.19685 G_PER_L |
CHEBI:132108
sodium silicate nonahydrate
|
|
| 10 |
Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
|
0.019685 G_PER_L |
CHEBI:53438
iron(3+) sulfate
|
|
| 11 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.000492126 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.000492126 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 14 |
CuSO4
Cu.O4S 159.609 g/mol
|
2 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
|
| 15 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 16 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 20 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.02 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 26 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 27 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 28 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 29 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 30 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 31 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 32 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components, adjust pH to 6.0 - 6.5 and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium:
- Distribute the medium into culture vessels (leave >80% volume gas phase) under a N2-CO2 (4:1) gas mixture and seal with butyl rubber stoppers. Pressurize the culture vessels to 200 kPa with methane gas. Finally add O2 gas to make up 2% O2 concentration in the gas phase. Readjust pH to 6.0 - 6.3, if necessary.
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.837416Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J524 |
| 2026-03-13T06:19:16.265853Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002875 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.787519+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANE OXIDIZING BACTERIUM MEDIUM" to "methane_oxidizing_bacterium_medium" for consistent matching |
| 2026-06-05T07:41:30.537041+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:06.597436+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |