acidianus_sp_jp7_medium

CultureMech:002876 archaea COMPLEX LIQUID
Original name: ACIDIANUS SP. JP7 MEDIUM

Overview

External ontology
mediadive.medium:J525 (source link unavailable) — ACIDIANUS SP. JP7 MEDIUM
Applications
  • Microbial cultivation
pH
0.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=525

Composition

flowchart LR
    CultureMech_002876["acidianus_sp_jp7_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002876 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002876 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002876 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002876 --> ing3_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing3_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing4_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002876 --> ing4_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing4_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing5_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_002876 --> ing5_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing5_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002876 --> ing6_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing7_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002876 --> ing7_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing7_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002876 --> ing8_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing9_VOSO4_x_n_H2O["VOSO4 x n H2O"]
    CultureMech_002876 --> ing9_VOSO4_x_n_H2O
    CHEBI_87020["CHEBI:87020"]
    ing9_VOSO4_x_n_H2O -.-> CHEBI_87020
    ing10_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002876 --> ing10_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing10_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing11_Yeast_extract["Yeast extract"]
    CultureMech_002876 --> ing11_Yeast_extract
    ing12_Chalcopyrite["Chalcopyrite"]
    CultureMech_002876 --> ing12_Chalcopyrite
    CHEBI_86202["CHEBI:86202"]
    ing12_Chalcopyrite -.-> CHEBI_86202

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.5 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.25 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.02 G_PER_L CHEBI:86254
iron trichloride hexahydrate
5 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
1.8 MG_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
6 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
4.5 MG_PER_L CHEBI:131366
disodium tetraborate decahydrate
7 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.22 MG_PER_L CHEBI:32312
zinc sulfate heptahydrate
8 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.05 MG_PER_L CHEBI:86318
copper(II) chloride dihydrate
9 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 MG_PER_L CHEBI:75213
sodium molybdate dihydrate
10 VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
0.03 MG_PER_L CHEBI:87020
vanadyl sulfate hydrate
11 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.01 MG_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
12 Yeast extract 0.1 G_PER_L
13 Chalcopyrite
Cu.Fe.2S 183.525 g/mol
10 G_PER_L CHEBI:86202
chalcopyrite

Preparation

  1. Dissolve ingredients except chalcopyrite in 500 ml distilled water, adjust pH to 0.8 with 10 N H2SO4 and autoclave. Pulp chalcopyrite in 500 ml distilled water and steam for 1 hr on each of 3 successive days. Combine the medium and the chalcopyrite concentrate.
  2. Comment: Chalcopyrite can be replaced with arsenopyrite or pyrite.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.842772Z mediadive-import Imported from MediaDive Source: JCM, ID: J525
2026-03-13T06:19:16.275230Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002876
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.798603+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDIANUS SP. JP7 MEDIUM" to "acidianus_sp_jp7_medium" for consistent matching
2026-06-05T07:40:47.093349+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.517508+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.