bacillus_filiformis_medium (CultureMech:002880)

Overview

External ontology

mediadive.medium:J531 (source link unavailable) — BACILLUS FILIFORMIS MEDIUM

Applications

Microbial cultivation

pH

9.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=531

Composition

flowchart LR
    CultureMech_002880["bacillus_filiformis_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002880 --> ing0_Yeast_extract
    ing1_Sodium_citrate["Sodium citrate"]
    CultureMech_002880 --> ing1_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing1_Sodium_citrate -.-> CHEBI_53258
    ing2_KCl["KCl"]
    CultureMech_002880 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_MgSO4_x_H2O["MgSO4 x H2O"]
    CultureMech_002880 --> ing3_MgSO4_x_H2O
    CHEBI_32599["CHEBI:32599"]
    ing3_MgSO4_x_H2O -.-> CHEBI_32599
    ing4_Na2CO3["Na2CO3"]
    CultureMech_002880 --> ing4_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing4_Na2CO3 -.-> CHEBI_29377
    ing5_NaCl["NaCl"]
    CultureMech_002880 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002880 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_FeSO4_x_H2O["FeSO4 x H2O"]
    CultureMech_002880 --> ing7_FeSO4_x_H2O
    CHEBI_75834["CHEBI:75834"]
    ing7_FeSO4_x_H2O -.-> CHEBI_75834

Ingredients (8)

#	Ingredient	Concentration	Ontology
1	Yeast extract	9.98004 G_PER_L
2	Sodium citrate C6H5O7.3Na 258.069 g/mol	2.99401 G_PER_L	CHEBI:53258 sodium citrate
3	KCl Cl.K 74.551 g/mol	1.99601 G_PER_L	CHEBI:32588 potassium chloride
4	MgSO4 x H2O Mg.O4S 120.368 g/mol	0.998004 G_PER_L	CHEBI:32599 magnesium sulfate
5	Na2CO3 CO3.2Na 105.988 g/mol	2.99401 G_PER_L	CHEBI:29377 sodium carbonate
6	NaCl Cl.Na 58.443 g/mol	99.8004 G_PER_L	CHEBI:26710 sodium chloride
7	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.36 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate
8	FeSO4 x H2O Fe.H2O.O4S 169.923 g/mol	50 G_PER_L	CHEBI:75834 iron(2+) sulfate monohydrate

Preparation

Separately autoclave Na2CO3 and NaCl. Check final pH to be about 9.0.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:16.853713Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J531
2026-03-13T06:19:16.289542Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002880
2026-03-17T06:30:07.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.816438+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "BACILLUS FILIFORMIS MEDIUM" to "bacillus_filiformis_medium" for consistent matching
2026-06-05T07:40:17.466792+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.034249+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.