Overview
- External ontology
- mediadive.medium:J535 (source link unavailable) — PY MEDIUM (B)
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=535
Composition
flowchart LR
CultureMech_002883["py_medium_b"]
ing0_Polypeptone["Polypeptone"]
CultureMech_002883 --> ing0_Polypeptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002883 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_002883 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002883 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002883 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002883 --> ing5_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing5_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing6_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002883 --> ing6_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing6_CuCl2_x_2_H2O -.-> CHEBI_86318
ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002883 --> ing7_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Polypeptone | 2 G_PER_L | ||
| 2 | Yeast extract | 0.5 G_PER_L | ||
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.005 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.005 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 7 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.004 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 8 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.006 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Preparation
- Adjust pH to 8.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.861959Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J535 |
| 2026-03-13T06:19:16.305046Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002883 |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:29.837088+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PY MEDIUM (B)" to "py_medium_b" for consistent matching |
| 2026-06-05T07:41:42.945886+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-07T14:52:40.011908+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |