mga_agar (CultureMech:002888)

Overview

External ontology

mediadive.medium:J53 (source link unavailable) — MGA AGAR

Applications

Microbial cultivation

pH

7.4

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=53

Composition

flowchart LR
    CultureMech_002888["mga_agar"]
    ing0_Glucose["Glucose"]
    CultureMech_002888 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_L_Asparagine["L-Asparagine"]
    CultureMech_002888 --> ing1_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing1_L_Asparagine -.-> CHEBI_17196
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002888 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002888 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Agar["Agar"]
    CultureMech_002888 --> ing4_Agar
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar -.-> CHEBI_2509

Ingredients (5)

#	Ingredient	Concentration	Ontology
1	Glucose C6H12O6 180.156 g/mol	1.998 G_PER_L	CHEBI:17234 glucose
2	L-Asparagine C4H8N2O3 132.119 g/mol	0.999001 G_PER_L	CHEBI:17196 L-asparagine
3	K2HPO4 HO4P.2K 174.174 g/mol	0.499501 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.499501 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
5	Agar	14.985 G_PER_L	CHEBI:2509 agar

Preparation

Adjust pH to 7.4.

Solutions

Trace salts solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
FeSO4 x 7 H2O	10 G_PER_L
CuSO4 x 5 H2O	1 G_PER_L
ZnSO4 x 7 H2O	1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:15.650511Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J53
2026-03-13T06:19:16.352319Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002888
2026-03-17T06:29:50.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.893462+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MGA AGAR" to "mga_agar" for consistent matching
2026-06-05T07:41:33.582840+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.735367+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.451840+00:00	apply_cocktail_nesting.py	NESTED_FLATTENED_COCKTAIL	Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace salts solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).