modified_syntrophomonas_medium_for_ol4

CultureMech:002889 bacterial DEFINED LIQUID
Original name: MODIFIED SYNTROPHOMONAS MEDIUM FOR OL4

Overview

External ontology
mediadive.medium:J540 (source link unavailable) — MODIFIED SYNTROPHOMONAS MEDIUM FOR OL4
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=540

Composition

flowchart LR
    CultureMech_002889["modified_syntrophomonas_medium_for_ol4"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_002889 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_Resazurin["Resazurin"]
    CultureMech_002889 --> ing1_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing1_Resazurin -.-> CHEBI_8806
    ing2_Sodium_oleate["Sodium oleate"]
    CultureMech_002889 --> ing2_Sodium_oleate
    CHEBI_81860["CHEBI:81860"]
    ing2_Sodium_oleate -.-> CHEBI_81860
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002889 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002889 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_NaCl["NaCl"]
    CultureMech_002889 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_NH4Cl["NH4Cl"]
    CultureMech_002889 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002889 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_HCl["HCl"]
    CultureMech_002889 --> ing8_HCl
    CHEBI_17883["CHEBI:17883"]
    ing8_HCl -.-> CHEBI_17883
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002889 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_ZnCl2["ZnCl2"]
    CultureMech_002889 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002889 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002889 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002889 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002889 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002889 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002889 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_Biotin["Biotin"]
    CultureMech_002889 --> ing17_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing17_Biotin -.-> CHEBI_15956
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002889 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002889 --> ing19_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing19_Thiamine_HCl -.-> CHEBI_49105
    ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002889 --> ing20_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing21_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002889 --> ing21_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing21_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002889 --> ing22_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 Na2SO4
2Na.O4S 142.043 g/mol
3.49127 G_PER_L CHEBI:32149
sodium sulfate
2 Resazurin
C12H7NO4 229.191 g/mol
0.00124688 G_PER_L CHEBI:8806
Resazurin
3 Sodium oleate
C18H33O2.Na 304.45 g/mol
1.5 G_PER_L CHEBI:81860
Sodium oleate
4 KH2PO4
H2O4P.K 136.084 g/mol
10 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
6.6 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 NaCl
Cl.Na 58.443 g/mol
8 G_PER_L CHEBI:26710
sodium chloride
7 NH4Cl
Cl.H4N 53.492 g/mol
8 G_PER_L CHEBI:31206
ammonium chloride
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
10 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
11 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 Biotin
C10H16N2O3S 244.316 g/mol
0.00025 G_PER_L CHEBI:15956
biotin
19 Nicotinic acid
C6H5NO2 123.111 g/mol
0.0025 G_PER_L CHEBI:15940
nicotinic acid
20 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.00125 G_PER_L CHEBI:49105
thiamine hydrochloride
21 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.00125 G_PER_L CHEBI:30753
4-aminobenzoic acid
22 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.00062 G_PER_L CHEBI:31345
Calcium pantothenate
23 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.0062 G_PER_L CHEBI:30961
pyridoxine hydrochloride

Preparation

  1. Mix components, adjust pH to 7.2, bring to a boil for a few seconds and cool down under a stream of a N2-CO2 (80:20) gas mixture. Distribute Solution A into culture vessels (e.g., 8 ml per tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave.
  2. Autoclave and store under a N2 atmosphere.
  3. To complete the medium, combine 0.8 volume of Solution A, 0.1 volume of Solution B and 0.1 volume of 3.5% NaHCO3 solution [filter-sterilized and stored under a N2-CO2 (80:20) gas mixture]. Reduce the medium with 0.3 g/L (final) L-cysteine x HCl x H2O and 0.3 g/L (final) Na2S x 9H2O (autoclave and store 3% stock solutions under a N2 atmosphere).
  4. Comment: For strain JCM 13948, incubate with a 10% inoculum together with a 5% inoculum of <I>Methanobacterium formicicum</I> NT (=JCM 10132).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.879745Z mediadive-import Imported from MediaDive Source: JCM, ID: J540
2026-03-13T06:19:16.361416Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002889
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.903549+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED SYNTROPHOMONAS MEDIUM FOR OL4" to "modified_syntrophomonas_medium_for_ol4" for consistent matching
2026-06-05T07:41:37.853904+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.981131+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.