desulfotomaculum_lam5_medium

CultureMech:002893 bacterial COMPLEX LIQUID
Original name: DESULFOTOMACULUM Lam5 MEDIUM

Overview

External ontology
mediadive.medium:J545 (source link unavailable) — DESULFOTOMACULUM Lam5 MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=545

Composition

flowchart LR
    CultureMech_002893["desulfotomaculum_lam5_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002893 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002893 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002893 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_002893 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Na2SO4["Na2SO4"]
    CultureMech_002893 --> ing4_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing4_Na2SO4 -.-> CHEBI_32149
    ing5_KCl["KCl"]
    CultureMech_002893 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2["CaCl2"]
    CultureMech_002893 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002893 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_002893 --> ing8_Yeast_extract
    ing9_Sodium_lactate["Sodium lactate"]
    CultureMech_002893 --> ing9_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing9_Sodium_lactate -.-> CHEBI_75228
    ing10_NaHCO3["NaHCO3"]
    CultureMech_002893 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002893 --> ing11_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002893 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_Resazurin["Resazurin"]
    CultureMech_002893 --> ing13_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Resazurin -.-> CHEBI_8806
    ing14_HCl["HCl"]
    CultureMech_002893 --> ing14_HCl
    CHEBI_17883["CHEBI:17883"]
    ing14_HCl -.-> CHEBI_17883
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002893 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_H3BO3["H3BO3"]
    CultureMech_002893 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002893 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002893 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002893 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002893 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002893 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002893 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.29703 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.29703 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.990099 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
0.990099 G_PER_L CHEBI:26710
sodium chloride
5 Na2SO4
2Na.O4S 142.043 g/mol
2.9703 G_PER_L CHEBI:32149
sodium sulfate
6 KCl
Cl.K 74.551 g/mol
0.0990099 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2
Ca.2Cl 110.984 g/mol
0.0990099 G_PER_L CHEBI:3312
calcium dichloride
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.49505 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 Yeast extract 0.0990099 G_PER_L
10 Sodium lactate
C3H5O3.Na 112.06 g/mol
0.990099 G_PER_L CHEBI:75228
sodium lactate
11 NaHCO3
CHO3.Na 84.006 g/mol
1.9802 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.49505 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
13 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.39604 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
14 Resazurin
C12H7NO4 229.191 g/mol
0.000990099 G_PER_L CHEBI:8806
Resazurin
15 HCl
HCl 36.461 g/mol
12.5 G_PER_L CHEBI:17883
hydrogen chloride
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.144 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O and adjust pH to 7.2. Distribute the medium into tubes (e.g., 5 ml of the medium in the Hungate tubes) under a N2 x CO2 gas mixture (4:1, v/v), seal with butyl rubber stoppers and autoclave. After cooling, add solutions of NaHCO3 [5%, filter-sterilized and stored under a N2-CO2 (4:1, v/v) gas mixture], L-cysteine x HCl x H2O and Na2S x 9H2O (5%, autoclaved and stored under N2) to the medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.895533Z mediadive-import Imported from MediaDive Source: JCM, ID: J545
2026-03-13T06:19:16.396630Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002893
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.942276+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOTOMACULUM Lam5 MEDIUM" to "desulfotomaculum_lam5_medium" for consistent matching
2026-06-05T07:40:57.575688+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.947831+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.