Overview
- External ontology
- mediadive.medium:J551 (source link unavailable) — DESULFOVIBRIO MARINUS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=551
Composition
flowchart LR
CultureMech_002899["desulfovibrio_marinus_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002899 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_002899 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_002899 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Na2SO4["Na2SO4"]
CultureMech_002899 --> ing3_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing3_Na2SO4 -.-> CHEBI_32149
ing4_NaCl["NaCl"]
CultureMech_002899 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_KCl["KCl"]
CultureMech_002899 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_CaCl2["CaCl2"]
CultureMech_002899 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_Resazurin["Resazurin"]
CultureMech_002899 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_Yeast_extract["Yeast extract"]
CultureMech_002899 --> ing8_Yeast_extract
ing9_Sodium_lactate["Sodium lactate"]
CultureMech_002899 --> ing9_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing9_Sodium_lactate -.-> CHEBI_75228
ing10_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002899 --> ing10_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing10_MgCl2_x_6_H2O -.-> CHEBI_86345
ing11_NaHCO3["NaHCO3"]
CultureMech_002899 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002899 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_HCl["HCl"]
CultureMech_002899 --> ing13_HCl
CHEBI_17883["CHEBI:17883"]
ing13_HCl -.-> CHEBI_17883
ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002899 --> ing14_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
ing15_H3BO3["H3BO3"]
CultureMech_002899 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002899 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002899 --> ing17_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002899 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002899 --> ing19_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002899 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002899 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.332594 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Copied from referenced JCM Medium 549 |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.332594 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Copied from referenced JCM Medium 549 |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.10865 G_PER_L |
CHEBI:31206
ammonium chloride
|
Copied from referenced JCM Medium 549 |
| 4 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.10421 G_PER_L |
CHEBI:32149
sodium sulfate
|
Copied from referenced JCM Medium 549 |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
11.0865 G_PER_L |
CHEBI:26710
sodium chloride
|
Copied from referenced JCM Medium 549 |
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.110865 G_PER_L |
CHEBI:32588
potassium chloride
|
Copied from referenced JCM Medium 549 |
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.110865 G_PER_L |
CHEBI:3312
calcium dichloride
|
Copied from referenced JCM Medium 549 |
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00110865 G_PER_L |
CHEBI:8806
Resazurin
|
Copied from referenced JCM Medium 549 |
| 9 | Yeast extract | 0.05 G_PER_L | Copied from referenced JCM Medium 549 | |
| 10 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.1 G_PER_L |
CHEBI:75228
sodium lactate
|
Copied from referenced JCM Medium 549 |
| 11 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Copied from referenced JCM Medium 549 |
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Copied from referenced JCM Medium 549 |
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.05 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Copied from referenced JCM Medium 549 |
| 14 |
HCl
HCl 36.461 g/mol
|
12.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
Copied from referenced JCM Medium 549 |
| 15 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Copied from referenced JCM Medium 549 |
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
Copied from referenced JCM Medium 549 |
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Copied from referenced JCM Medium 549 |
| 18 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Copied from referenced JCM Medium 549 |
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
Copied from referenced JCM Medium 549 |
| 20 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Copied from referenced JCM Medium 549 |
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Copied from referenced JCM Medium 549 |
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Copied from referenced JCM Medium 549 |
Preparation
- Use Medium No. 549 supplemented with final 50.0 g/L NaCl.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.912946Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J551 |
| 2026-02-03T11:26:55.287250 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:16.434046Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002899 |
| 2026-03-17T19:45:29.984657+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOVIBRIO MARINUS MEDIUM" to "desulfovibrio_marinus_medium" for consistent matching |
| 2026-04-04T18:49:19.771739 | copy_referenced_compositions | RESOLVED_REFERENCE | Resolved reference by copying from desulfovibrio_as36_medium (CultureMech:002896) |
| 2026-05-16T08:57:28.766451+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(2 keys) -> list(1 entries) |
| 2026-06-05T07:40:57.871336+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.062411+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |