sulfate_reducing_bacterium_medium_with_lactate

CultureMech:002900 bacterial COMPLEX LIQUID
Original name: SULFATE REDUCING BACTERIUM MEDIUM WITH LACTATE

Overview

External ontology
mediadive.medium:J552 (source link unavailable) — SULFATE REDUCING BACTERIUM MEDIUM WITH LACTATE
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=552

Composition

flowchart LR
    CultureMech_002900["sulfate_reducing_bacterium_medium_with_lactate"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002900 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002900 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Na2SO4["Na2SO4"]
    CultureMech_002900 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002900 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002900 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_002900 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002900 --> ing6_Yeast_extract
    ing7_L_Sodium_lactate["L-Sodium lactate"]
    CultureMech_002900 --> ing7_L_Sodium_lactate
    CHEBI_232798["CHEBI:232798"]
    ing7_L_Sodium_lactate -.-> CHEBI_232798
    ing8_Resazurin["Resazurin"]
    CultureMech_002900 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002900 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Biotin["Biotin"]
    CultureMech_002900 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002900 --> ing11_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing11_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing12_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002900 --> ing12_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing12_Nicotinic_acid -.-> CHEBI_15940
    ing13_HCl["HCl"]
    CultureMech_002900 --> ing13_HCl
    CHEBI_17883["CHEBI:17883"]
    ing13_HCl -.-> CHEBI_17883
    ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002900 --> ing14_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing15_ZnCl2["ZnCl2"]
    CultureMech_002900 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002900 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_H3BO3["H3BO3"]
    CultureMech_002900 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002900 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002900 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002900 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002900 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.498504 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.997009 G_PER_L CHEBI:31206
ammonium chloride
3 Na2SO4
2Na.O4S 142.043 g/mol
0.997009 G_PER_L CHEBI:32149
sodium sulfate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0997009 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1.99402 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 NaCl
Cl.Na 58.443 g/mol
9.97009 G_PER_L CHEBI:26710
sodium chloride
7 Yeast extract 0.498504 G_PER_L
8 L-Sodium lactate
C3H5O3.Na 112.06 g/mol
2.29312 G_PER_L CHEBI:232798
sodium L-lactate
9 Resazurin
C12H7NO4 229.191 g/mol
0.000997009 G_PER_L CHEBI:8806
Resazurin
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.498504 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
11 Biotin
C10H16N2O3S 244.316 g/mol
10 G_PER_L CHEBI:15956
biotin
12 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
40 G_PER_L CHEBI:30753
4-aminobenzoic acid
13 Nicotinic acid
C6H5NO2 123.111 g/mol
100 G_PER_L CHEBI:15940
nicotinic acid
14 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
15 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
17 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients except L-sodium lactate and L-cysteine x H2O x HCl, adjust pH to 7.5 and autoclave under a N2 atmosphere. Separately autoclave L-sodium lactate and L-cysteine x H2O x HCl in 10 ml distilled water under a N2 atmosphere and add to the medium. Readjust pH to 7.5 with 1 N NaOH (from a sterile stock solution).
  2. For preparation of solid medium, add 15 g/L agar (BD-Difco).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 2 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-26T17:53:16.914243Z mediadive-import Imported from MediaDive Source: JCM, ID: J552
2026-03-13T06:19:16.441657Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002900
2026-03-17T06:30:21.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:29.992774+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SULFATE REDUCING BACTERIUM MEDIUM WITH LACTATE" to "sulfate_reducing_bacterium_medium_with_lactate" for consistent matching
2026-06-05T07:41:47.324809+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.965307+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.