Overview
- External ontology
- mediadive.medium:J561 (source link unavailable) — THIORHODOCOCCUS BHEEMLICUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=561
Composition
flowchart LR
CultureMech_002910["thiorhodococcus_bheemlicum_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002910 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002910 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002910 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NH4Cl["NH4Cl"]
CultureMech_002910 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_NaCl["NaCl"]
CultureMech_002910 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Yeast_extract["Yeast extract"]
CultureMech_002910 --> ing5_Yeast_extract
ing6_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002910 --> ing6_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing6_Sodium_pyruvate -.-> CHEBI_50144
ing7_Ferric_citrate["Ferric citrate"]
CultureMech_002910 --> ing7_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing7_Ferric_citrate -.-> CHEBI_144421
ing8_NaHCO3["NaHCO3"]
CultureMech_002910 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002910 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002910 --> ing10_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing10_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing11_HCl["HCl"]
CultureMech_002910 --> ing11_HCl
CHEBI_17883["CHEBI:17883"]
ing11_HCl -.-> CHEBI_17883
ing12_ZnCl2["ZnCl2"]
CultureMech_002910 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002910 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_002910 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002910 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002910 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002910 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002910 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.489716 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.146915 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.95886 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.333007 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
19.5886 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 | Yeast extract | 0.391773 G_PER_L | ||
| 7 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
2.9383 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 8 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
5 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
15 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.240182 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1.48912 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 12 |
HCl
HCl 36.461 g/mol
|
1 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0699301 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0999001 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.0599401 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.1998 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01998 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.01998 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03996 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.0.
- Autoclave except NaHCO3, Na2S x 9H2O and Na2S2O3 x 5H2O. After cooling, aseptically add filter-sterilized NaHCO3, Na2S x 9H2O and Na2S2O3 x 5H2O solutions to the medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.939420Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J561 |
| 2026-03-13T06:19:16.507216Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002910 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.068347+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THIORHODOCOCCUS BHEEMLICUM MEDIUM" to "thiorhodococcus_bheemlicum_medium" for consistent matching |
| 2026-06-05T07:41:51.719968+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:13.606739+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |