desulfovibrio_heb233_medium

CultureMech:002914 bacterial COMPLEX LIQUID
Original name: DESULFOVIBRIO HEB233 MEDIUM

Overview

External ontology
mediadive.medium:J565 (source link unavailable) — DESULFOVIBRIO HEB233 MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=565

Composition

flowchart LR
    CultureMech_002914["desulfovibrio_heb233_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002914 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002914 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002914 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_Na2SO4["Na2SO4"]
    CultureMech_002914 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_NaCl["NaCl"]
    CultureMech_002914 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_KCl["KCl"]
    CultureMech_002914 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2["CaCl2"]
    CultureMech_002914 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002914 --> ing7_Yeast_extract
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002914 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Resazurin["Resazurin"]
    CultureMech_002914 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Sodium_lactate["Sodium lactate"]
    CultureMech_002914 --> ing10_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing10_Sodium_lactate -.-> CHEBI_75228
    ing11_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002914 --> ing11_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing11_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing12_NaHCO3["NaHCO3"]
    CultureMech_002914 --> ing12_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing12_NaHCO3 -.-> CHEBI_32139
    ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002914 --> ing13_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing13_Na2S_x_9_H2O -.-> CHEBI_76209
    ing14_HCl["HCl"]
    CultureMech_002914 --> ing14_HCl
    CHEBI_17883["CHEBI:17883"]
    ing14_HCl -.-> CHEBI_17883
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002914 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_H3BO3["H3BO3"]
    CultureMech_002914 --> ing16_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing16_H3BO3 -.-> CHEBI_33118
    ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002914 --> ing17_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002914 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002914 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002914 --> ing20_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002914 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002914 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (23)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.332594 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.332594 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
1.10865 G_PER_L CHEBI:31206
ammonium chloride
4 Na2SO4
2Na.O4S 142.043 g/mol
3.32594 G_PER_L CHEBI:32149
sodium sulfate
5 NaCl
Cl.Na 58.443 g/mol
25.4989 G_PER_L CHEBI:26710
sodium chloride
6 KCl
Cl.K 74.551 g/mol
0.110865 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2
Ca.2Cl 110.984 g/mol
0.110865 G_PER_L CHEBI:3312
calcium dichloride
8 Yeast extract 0.110865 G_PER_L
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.554324 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Resazurin
C12H7NO4 229.191 g/mol
0.00110865 G_PER_L CHEBI:8806
Resazurin
11 Sodium lactate
C3H5O3.Na 112.06 g/mol
0.1 G_PER_L CHEBI:75228
sodium lactate
12 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.1 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
13 NaHCO3
CHO3.Na 84.006 g/mol
0.2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
14 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.1 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
15 HCl
HCl 36.461 g/mol
12.5 G_PER_L CHEBI:17883
hydrogen chloride
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
17 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
18 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
21 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
22 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.144 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients and adjust pH to 7.2. Distribute 0.9 volume of the medium into tubes (e.g., 4.5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 45 min.
  2. To complete the medium, add the following stock solutions [Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere] to the medium (per 4.5 ml):

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:16.948839Z mediadive-import Imported from MediaDive Source: JCM, ID: J565
2026-03-13T06:19:16.528725Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002914
2026-03-17T06:30:31.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.092067+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOVIBRIO HEB233 MEDIUM" to "desulfovibrio_heb233_medium" for consistent matching
2026-06-05T07:40:57.816972+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.041082+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.