Overview
- External ontology
- mediadive.medium:J566 (source link unavailable) — ILYOBACTER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=566
Composition
flowchart LR
CultureMech_002915["ilyobacter_medium"]
ing0_NaCl["NaCl"]
CultureMech_002915 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_002915 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002915 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002915 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_NH4Cl["NH4Cl"]
CultureMech_002915 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KNO3["KNO3"]
CultureMech_002915 --> ing5_KNO3
CHEBI_63043["CHEBI:63043"]
ing5_KNO3 -.-> CHEBI_63043
ing6_KH2PO4_x_2_H2O["KH2PO4 x 2 H2O"]
CultureMech_002915 --> ing6_KH2PO4_x_2_H2O
ing7_Yeast_extract["Yeast extract"]
CultureMech_002915 --> ing7_Yeast_extract
ing8_Peptone["Peptone"]
CultureMech_002915 --> ing8_Peptone
ing9_Sodium_acetate["Sodium acetate"]
CultureMech_002915 --> ing9_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Sodium_acetate -.-> CHEBI_32954
ing10_Sodium_succinate["Sodium succinate"]
CultureMech_002915 --> ing10_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing10_Sodium_succinate -.-> CHEBI_63675
ing11_DL_Sodium_malate["DL-Sodium malate"]
CultureMech_002915 --> ing11_DL_Sodium_malate
CHEBI_91260["CHEBI:91260"]
ing11_DL_Sodium_malate -.-> CHEBI_91260
ing12_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002915 --> ing12_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing12_Sodium_pyruvate -.-> CHEBI_50144
ing13_Glucose["Glucose"]
CultureMech_002915 --> ing13_Glucose
CHEBI_17234["CHEBI:17234"]
ing13_Glucose -.-> CHEBI_17234
ing14_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002915 --> ing14_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing14_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing15_Resazurin["Resazurin"]
CultureMech_002915 --> ing15_Resazurin
CHEBI_8806["CHEBI:8806"]
ing15_Resazurin -.-> CHEBI_8806
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002915 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2SeO3["Na2SeO3"]
CultureMech_002915 --> ing17_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing17_Na2SeO3 -.-> CHEBI_48843
ing18_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002915 --> ing18_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing18_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing19_Biotin["Biotin"]
CultureMech_002915 --> ing19_Biotin
CHEBI_15956["CHEBI:15956"]
ing19_Biotin -.-> CHEBI_15956
ing20_Folic_acid["Folic acid"]
CultureMech_002915 --> ing20_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing20_Folic_acid -.-> CHEBI_27470
ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002915 --> ing21_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing22_Thiamine_HCl["Thiamine HCl"]
CultureMech_002915 --> ing22_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing22_Thiamine_HCl -.-> CHEBI_49105
ing23_Riboflavin["Riboflavin"]
CultureMech_002915 --> ing23_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing23_Riboflavin -.-> CHEBI_17015
ing24_Nicotinic_acid["Nicotinic acid"]
CultureMech_002915 --> ing24_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing24_Nicotinic_acid -.-> CHEBI_15940
ing25_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002915 --> ing25_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing25_Calcium_pantothenate -.-> CHEBI_31345
ing26_Vitamin_B12["Vitamin B12"]
CultureMech_002915 --> ing26_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing26_Vitamin_B12 -.-> CHEBI_176843
ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002915 --> ing27_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
ing28_Lipoic_acid["Lipoic acid"]
CultureMech_002915 --> ing28_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing28_Lipoic_acid -.-> CHEBI_16494
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
9.90099 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.381188 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.00990099 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.247525 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.49505 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KNO3
K.NO3 101.102 g/mol
|
0.49505 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 7 | KH2PO4 x 2 H2O | 0.247525 G_PER_L | ||
| 8 | Yeast extract | 0.247525 G_PER_L | ||
| 9 | Peptone | 0.247525 G_PER_L | ||
| 10 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.049505 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 11 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.049505 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 12 | DL-Sodium malate | 0.049505 G_PER_L |
CHEBI:91260
disodium malate
|
|
| 13 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.049505 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 14 |
Glucose
C6H12O6 180.156 g/mol
|
0.49505 G_PER_L |
CHEBI:17234
glucose
|
|
| 15 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.148515 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 16 |
Resazurin
C12H7NO4 229.191 g/mol
|
100 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.001 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 19 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.01 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 20 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 21 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 22 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 23 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 24 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 25 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 26 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 27 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 28 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 29 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Adjust pH to 7.0.
- Boil until the volume decreases to 1.0 L and dispense under an atmosphere of N2 into 15 ml Hungate tube. If the medium stays pink (O2 is present), add one drop of 5% Na2S from the sterile stock solution.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.952552Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J566 |
| 2026-03-13T06:19:16.540472Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002915 |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.105509+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ILYOBACTER MEDIUM" to "ilyobacter_medium" for consistent matching |
| 2026-06-05T07:41:09.322990+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.002852+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:00.029476+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |