desulfohimalaya_medium

CultureMech:002934 bacterial COMPLEX LIQUID
Original name: DESULFOHIMALAYA MEDIUM

Overview

External ontology
mediadive.medium:J587 (source link unavailable) — DESULFOHIMALAYA MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=587

Composition

flowchart LR
    CultureMech_002934["desulfohimalaya_medium"]
    ing0_Sodium_lactate["Sodium lactate"]
    CultureMech_002934 --> ing0_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing0_Sodium_lactate -.-> CHEBI_75228
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002934 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002934 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_002934 --> ing3_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing3_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_002934 --> ing4_Yeast_extract
    ing5_KH2PO4["KH2PO4"]
    CultureMech_002934 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002934 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_Ascorbic_acid["Ascorbic acid"]
    CultureMech_002934 --> ing7_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing7_Ascorbic_acid -.-> CHEBI_22652
    ing8_Thioglycollic_acid["Thioglycollic acid"]
    CultureMech_002934 --> ing8_Thioglycollic_acid
    CHEBI_30065["CHEBI:30065"]
    ing8_Thioglycollic_acid -.-> CHEBI_30065

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Sodium lactate
C3H5O3.Na 112.06 g/mol
3.5 G_PER_L CHEBI:75228
sodium lactate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
4 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
1 G_PER_L CHEBI:32583
calcium sulfate dihydrate
5 Yeast extract 1 G_PER_L
6 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.5 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 Ascorbic acid 0.1 G_PER_L CHEBI:22652
ascorbic acid
9 Thioglycollic acid
C2H4O2S 92.119 g/mol
0.1 G_PER_L CHEBI:30065
thioglycolic acid

Preparation

  1. Adjust pH to 7.3.
  2. Add components except ascorbic acid and thioglycollic acid to distilled water and adjust pH to 7.0 - 7.5. Thioglycollate and ascorbate should be added immediately prior to sterilization. Distribute into tubes or flasks. This medium may be cloudy after sterilization and turns black as a result of H2S production due to bacterial growth.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.010935Z mediadive-import Imported from MediaDive Source: JCM, ID: J587
2026-03-13T06:19:16.659846Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002934
2026-03-17T06:30:07.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.240727+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOHIMALAYA MEDIUM" to "desulfohimalaya_medium" for consistent matching
2026-06-05T07:40:56.759529+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.637938+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-20T19:01:40.148055+00:00 apply_mim_groundings.py Adopted MIM published grounding Grounded 1 ingredient(s) from MIM's ingredient_mappings.sssom.tsv (2026-08-18), exactMatch only, where this record carried no CHEBI term (#308).
2026-10-07T14:52:40.011908+00:00 codex CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.