modified_rhodobacter_spaeroides_medium

CultureMech:002935 bacterial COMPLEX LIQUID
Original name: MODIFIED RHODOBACTER SPAEROIDES MEDIUM

Overview

External ontology
mediadive.medium:J588 (source link unavailable) — MODIFIED RHODOBACTER SPAEROIDES MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=588

Composition

flowchart LR
    CultureMech_002935["modified_rhodobacter_spaeroides_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002935 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002935 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_NaCl["NaCl"]
    CultureMech_002935 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002935 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002935 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Sorbitol["Sorbitol"]
    CultureMech_002935 --> ing5_Sorbitol
    CHEBI_30911["CHEBI:30911"]
    ing5_Sorbitol -.-> CHEBI_30911
    ing6_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002935 --> ing6_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Sodium_pyruvate -.-> CHEBI_50144
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002935 --> ing7_Yeast_extract
    ing8_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_002935 --> ing8_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing8_Fe_III__citrate -.-> CHEBI_144421
    ing9_HCl["HCl"]
    CultureMech_002935 --> ing9_HCl
    CHEBI_17883["CHEBI:17883"]
    ing9_HCl -.-> CHEBI_17883
    ing10_ZnCl2["ZnCl2"]
    CultureMech_002935 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002935 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002935 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002935 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002935 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002935 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002935 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.497018 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.198807 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 NaCl
Cl.Na 58.443 g/mol
0.397614 G_PER_L CHEBI:26710
sodium chloride
4 NH4Cl
Cl.H4N 53.492 g/mol
0.397614 G_PER_L CHEBI:31206
ammonium chloride
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0497018 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 Sorbitol
C6H14O6 182.172 g/mol
2.98211 G_PER_L CHEBI:30911
glucitol
7 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
2.98211 G_PER_L CHEBI:50144
sodium pyruvate
8 Yeast extract 0.596421 G_PER_L
9 Fe(III) citrate
C6H5FeO7 244.944 g/mol
5 G_PER_L CHEBI:144421
iron(III) citrate
10 HCl
HCl 36.461 g/mol
1 G_PER_L CHEBI:17883
hydrogen chloride
11 ZnCl2
2Cl.Zn 136.296 g/mol
0.0699301 G_PER_L CHEBI:49976
zinc dichloride
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0999001 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 H3BO3
H3BO3 61.833 g/mol
0.0599401 G_PER_L CHEBI:33118
boric acid
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.1998 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01998 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01998 G_PER_L CHEBI:34887
nickel dichloride
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03996 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 7.0.
  2. Add the following component after autoclaving:

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.013917Z mediadive-import Imported from MediaDive Source: JCM, ID: J588
2026-03-13T06:19:16.666955Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002935
2026-03-17T06:30:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.249206+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED RHODOBACTER SPAEROIDES MEDIUM" to "modified_rhodobacter_spaeroides_medium" for consistent matching
2026-06-05T07:41:37.701104+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.929877+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.