Overview
- External ontology
- mediadive.medium:J589 (source link unavailable) — PY MEDIUM (C)
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=589
Composition
flowchart LR
CultureMech_002936["py_medium_c"]
ing0_Peptone["Peptone"]
CultureMech_002936 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002936 --> ing1_Yeast_extract
ing2_Na2CO3["Na2CO3"]
CultureMech_002936 --> ing2_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing2_Na2CO3 -.-> CHEBI_29377
ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002936 --> ing3_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing3_Na2S_x_9_H2O -.-> CHEBI_76209
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002936 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002936 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_KH2PO4["KH2PO4"]
CultureMech_002936 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_K2HPO4["K2HPO4"]
CultureMech_002936 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_NaHCO3["NaHCO3"]
CultureMech_002936 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_NaCl["NaCl"]
CultureMech_002936 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 9.43396 G_PER_L | ||
| 2 | Yeast extract | 9.43396 G_PER_L | ||
| 3 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 4 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- Mix components thoroughly and autoclave under a N2 atmosphere. Aseptically and anaerobically add the following solutions (autoclaved under a N2 atmosphere) to the medium:
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.017543Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J589 |
| 2026-03-13T06:19:16.672825Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002936 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.256760+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PY MEDIUM (C)" to "py_medium_c" for consistent matching |
| 2026-06-05T07:41:42.951731+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.555009+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |