g5g6_medium (CultureMech:002948)

Overview

External ontology

mediadive.medium:J600 (source link unavailable) — G5G6 MEDIUM

Applications

Microbial cultivation

pH

7.3

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=600

Composition

flowchart LR
    CultureMech_002948["g5g6_medium"]
    ing0_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_002948 --> ing0_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing0_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002948 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Resazurin["Resazurin"]
    CultureMech_002948 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002948 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002948 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_NH4HCO3["NH4HCO3"]
    CultureMech_002948 --> ing5_NH4HCO3
    CHEBI_184335["CHEBI:184335"]
    ing5_NH4HCO3 -.-> CHEBI_184335
    ing6_NaHCO3["NaHCO3"]
    CultureMech_002948 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002948 --> ing7_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing8_NaNO3["NaNO3"]
    CultureMech_002948 --> ing8_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing8_NaNO3 -.-> CHEBI_63005
    ing9_Toluene["Toluene"]
    CultureMech_002948 --> ing9_Toluene
    CHEBI_17578["CHEBI:17578"]
    ing9_Toluene -.-> CHEBI_17578
    ing10_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
    CultureMech_002948 --> ing10_AlCl3_x_6_H2O
    CHEBI_30115["CHEBI:30115"]
    ing10_AlCl3_x_6_H2O -.-> CHEBI_30115
    ing11_HCl["HCl"]
    CultureMech_002948 --> ing11_HCl
    CHEBI_17883["CHEBI:17883"]
    ing11_HCl -.-> CHEBI_17883
    ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002948 --> ing12_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing13_Biotin["Biotin"]
    CultureMech_002948 --> ing13_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing13_Biotin -.-> CHEBI_15956
    ing14_Folic_acid["Folic acid"]
    CultureMech_002948 --> ing14_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing14_Folic_acid -.-> CHEBI_27470
    ing15_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002948 --> ing15_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing15_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing16_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002948 --> ing16_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing16_Thiamine_HCl -.-> CHEBI_49105
    ing17_Riboflavin["Riboflavin"]
    CultureMech_002948 --> ing17_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing17_Riboflavin -.-> CHEBI_17015
    ing18_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002948 --> ing18_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing18_Nicotinic_acid -.-> CHEBI_15940
    ing19_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002948 --> ing19_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing19_Calcium_pantothenate -.-> CHEBI_31345
    ing20_Vitamin_B12["Vitamin B12"]
    CultureMech_002948 --> ing20_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing20_Vitamin_B12 -.-> CHEBI_176843
    ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002948 --> ing21_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing22_Lipoic_acid["Lipoic acid"]
    CultureMech_002948 --> ing22_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing22_Lipoic_acid -.-> CHEBI_16494
    ing23_ZnCl2["ZnCl2"]
    CultureMech_002948 --> ing23_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing23_ZnCl2 -.-> CHEBI_49976
    ing24_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002948 --> ing24_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing24_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing25_H3BO3["H3BO3"]
    CultureMech_002948 --> ing25_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing25_H3BO3 -.-> CHEBI_33118
    ing26_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002948 --> ing26_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing26_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing27_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002948 --> ing27_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing27_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing28_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002948 --> ing28_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing28_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing29_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002948 --> ing29_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing29_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (30)

#	Ingredient	Concentration	Ontology	Notes
1	Na2HPO4 x 12 H2O HO4P.2Na 141.958 g/mol	0.566893 G_PER_L	CHEBI:34683 disodium hydrogenphosphate
2	KH2PO4 H2O4P.K 136.084 g/mol	0.736961 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
3	Resazurin C12H7NO4 229.191 g/mol	0.000566893 G_PER_L	CHEBI:8806 Resazurin
4	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	2.2 G_PER_L	CHEBI:86158 calcium chloride dihydrate
5	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	2 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
6	NH4HCO3 CHO3.H4N 79.055 g/mol	3.96396 G_PER_L	CHEBI:184335 Ammonium bicarbonate
7	NaHCO3 CHO3.Na 84.006 g/mol	33.6036 G_PER_L	CHEBI:32139 sodium hydrogencarbonate
8	L-Cysteine HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	1.57658 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate
9	NaNO3 NO3.Na 84.994 g/mol	10 G_PER_L	CHEBI:63005 sodium nitrate
10	Toluene C7H8 92.141 g/mol	40 G_PER_L	CHEBI:17578 toluene
11	AlCl3 x 6 H2O AlCl3.6H2O 241.431 g/mol	0.01 G_PER_L	CHEBI:30115 aluminium trichloride hexahydrate
12	HCl HCl 36.461 g/mol	2.5 G_PER_L	CHEBI:17883 hydrogen chloride
13	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	1.5 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate
14	Biotin C10H16N2O3S 244.316 g/mol	0.002 G_PER_L	CHEBI:15956 biotin
15	Folic acid C19H19N7O6 441.404 g/mol	0.002 G_PER_L	CHEBI:27470 folic acid
16	Pyridoxine hydrochloride C8H11NO3.HCl 205.641 g/mol	0.01 G_PER_L	CHEBI:30961 pyridoxine hydrochloride
17	Thiamine HCl C12H17N4OS.Cl.HCl 337.276 g/mol	0.005 G_PER_L	CHEBI:49105 thiamine hydrochloride
18	Riboflavin C17H20N4O6 376.369 g/mol	0.005 G_PER_L	CHEBI:17015 riboflavin
19	Nicotinic acid C6H5NO2 123.111 g/mol	0.005 G_PER_L	CHEBI:15940 nicotinic acid
20	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.005 G_PER_L	CHEBI:31345 Calcium pantothenate
21	Vitamin B12	0.050100000000000006 G_PER_L	CHEBI:176843 vitamin B12	[Merged 2 duplicates: 0.0001, 0.05]
22	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.005 G_PER_L	CHEBI:30753 4-aminobenzoic acid
23	Lipoic acid C8H14O2S2 206.332 g/mol	0.005 G_PER_L	CHEBI:16494 lipoic acid
24	ZnCl2 2Cl.Zn 136.296 g/mol	0.07 G_PER_L	CHEBI:49976 zinc dichloride
25	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.1 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate
26	H3BO3 H3BO3 61.833 g/mol	0.006 G_PER_L	CHEBI:33118 boric acid
27	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.19 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate
28	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.002 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate
29	NiCl2 x 6 H2O Cl2Ni 129.599 g/mol	0.024 G_PER_L	CHEBI:34887 nickel dichloride
30	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.036 G_PER_L	CHEBI:75213 sodium molybdate dihydrate

Preparation

Prepare all solutions and autoclave under a N2 atmosphere. After cooling, combine the solutions and replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture. Then, add the following autoclaved or *filter-sterilized solutions to the medium:
Check the final pH to be around 7.3.
Distribute appropriate amount of the medium into culture vessels under the same gas mixture (e.g., 40.0 ml in 120 ml serum bottle) and seal with Viton rubber stoppers. Finally add per liter the following solution from an anaerobic stock (autoclaved):
Comment: For strain JCM 14632, incubate with a 5-10% inoculum. When toluene is depleted, re-add the toluene solution (normally every 3 to 4 days, until enough cell growth is seen).

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:17.049727Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J600
2026-01-28T19:48:36.830871Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:33.329017Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 1 duplicate ingredient(s)
2026-03-13T06:19:16.753639Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002948
2026-03-17T06:30:43.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.351290+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "G5G6 MEDIUM" to "g5g6_medium" for consistent matching
2026-06-05T07:41:05.830951+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:58.914607+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.