halomonas_medium (CultureMech:002955)

Overview

External ontology

mediadive.medium:J608 (source link unavailable) — HALOMONAS MEDIUM

Applications

Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=608

Composition

flowchart LR
    CultureMech_002955["halomonas_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002955 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Casamino_acids["Casamino acids"]
    CultureMech_002955 --> ing1_Casamino_acids
    ing2_Proteose_peptone_no__3["Proteose peptone no. 3"]
    CultureMech_002955 --> ing2_Proteose_peptone_no__3
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002955 --> ing3_Yeast_extract
    ing4_Sodium_citrate["Sodium citrate"]
    CultureMech_002955 --> ing4_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing4_Sodium_citrate -.-> CHEBI_53258
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002955 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_K2HPO4["K2HPO4"]
    CultureMech_002955 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002955 --> ing7_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing7_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181

Ingredients (8)

#	Ingredient	Concentration	Ontology
1	NaCl Cl.Na 58.443 g/mol	80 G_PER_L	CHEBI:26710 sodium chloride
2	Casamino acids	7.5 G_PER_L
3	Proteose peptone no. 3	5 G_PER_L
4	Yeast extract	1 G_PER_L
5	Sodium citrate C6H5O7.3Na 258.069 g/mol	3 G_PER_L	CHEBI:53258 sodium citrate
6	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	7 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
7	K2HPO4 HO4P.2K 174.174 g/mol	0.5 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
8	Fe(NH4)2(SO4)2 x 6 H2O Fe.6H2O.2H4N.2O4S 392.139 g/mol	0.05 G_PER_L	CHEBI:76181 ferrous ammonium sulfate hexahydrate

Preparation

Adjust pH to 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:17.079661Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J608
2026-01-28T19:48:06.810948Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:16.813126Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002955
2026-03-17T06:29:39.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.418782+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "HALOMONAS MEDIUM" to "halomonas_medium" for consistent matching
2026-06-05T07:40:17.512314+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.373827+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:43.046738+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:52:40.011908+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.