glycerol_asparagine_agar_isp_5

CultureMech:002957 bacterial DEFINED SOLID_AGAR
Original name: GLYCEROL-ASPARAGINE AGAR (ISP-5)

Overview

External ontology
mediadive.medium:J60 (source link unavailable) — GLYCEROL-ASPARAGINE AGAR (ISP-5)
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: JCM Medium 60 | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=60 JCM Medium 60 Glycerol-Asparagine Agar (ISP-5) combines L-asparagine, glycerol, K2HPO4, 1 mL/L Trace salts solution, agar, and distilled water, adjusts the complete medium to pH 7.4, and autoclaves it.

Composition

flowchart LR
    CultureMech_002957["glycerol_asparagine_agar_isp_5"]
    ing0_L_Asparagine["L-Asparagine"]
    CultureMech_002957 --> ing0_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing0_L_Asparagine -.-> CHEBI_17196
    ing1_Glycerol["Glycerol"]
    CultureMech_002957 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002957 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Agar["Agar"]
    CultureMech_002957 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_002957 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 L-Asparagine
C4H8N2O3 132.119 g/mol
1.0 G_PER_L CHEBI:17196
L-asparagine
 JCM Medium 60 lists this component for Glycerol-Asparagine Agar (ISP-5).
2 Glycerol
C3H8O3 92.094 g/mol
10.0 G_PER_L CHEBI:17754
glycerol
 JCM Medium 60 lists this component for Glycerol-Asparagine Agar (ISP-5).
3 K2HPO4
HO4P.2K 174.174 g/mol
1.0 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 JCM Medium 60 lists this component for Glycerol-Asparagine Agar (ISP-5).
4 Agar 15.0 G_PER_L CHEBI:2509
agar
 JCM Medium 60 lists this component for Glycerol-Asparagine Agar (ISP-5).
5 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 JCM Medium 60 lists this distilled-water volume.

Preparation

  1. Dissolve the Glycerol-Asparagine Agar components in distilled water, add 1 mL Trace salts solution, and bring the final volume to 1 L.
  2. Adjust the complete medium to pH 7.4.
  3. Autoclave at 121 C for 15 min.

Solutions

Trace salts solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L JCM Medium 60 lists this trace-salt stock component.
MnCl2 x 4 H2O 1 G_PER_L JCM Medium 60 lists this trace-salt stock component.
ZnSO4 x 7 H2O 1 G_PER_L JCM Medium 60 lists this trace-salt stock component.
JCM Medium 60 prepares Trace salts solution from 0.1 g each FeSO4 x 7 H2O, MnCl2 x 4 H2O, and ZnSO4 x 7 H2O in 100 mL distilled water, then adds 1 mL stock per final liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:15.663197Z mediadive-import Imported from MediaDive Source: JCM, ID: J60
2026-03-13T06:19:16.820449Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002957
2026-03-17T06:30:40.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.427663+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GLYCEROL-ASPARAGINE AGAR (ISP-5)" to "glycerol_asparagine_agar_isp_5" for consistent matching
2026-06-05T07:41:06.539918+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.135487+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T07:04:21.829232+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace salt solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).
2026-09-08T00:00:00-07:00 repair_jcm_isp5_salinity_score20.py RESOLVED_JCM_ISP5_SALINITY_SCORE20 Rebuilt the JCM Medium 60 Glycerol-Asparagine Agar (ISP-5) recipe.
2026-09-08T00:00:00-07:00 repair_jcm_isp5_salinity_score20.py LINKED_JCM_ISP5_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/glycerol_asparagine_agar_isp_5_with_10_nacl.yaml.