sphaerotilus_medium

CultureMech:002958 bacterial COMPLEX SOLID_AGAR
Original name: SPHAEROTILUS MEDIUM

Overview

External ontology
mediadive.medium:J610 (source link unavailable) — SPHAEROTILUS MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
JCM Medium 610 Sphaerotilus Medium lists 0.5 g Tryptone (BD-Difco), 1.0 g Glycerol, 0.1 g Yeast extract (BD-Difco), 10.0 g Agar if needed, and 1.0 L Distilled water, adjusted to pH 7.0.

Composition

flowchart LR
    CultureMech_002958["sphaerotilus_medium"]
    ing0_Tryptone__BD_Difco_["Tryptone (BD-Difco)"]
    CultureMech_002958 --> ing0_Tryptone__BD_Difco_
    ing1_Glycerol["Glycerol"]
    CultureMech_002958 --> ing1_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing1_Glycerol -.-> CHEBI_17754
    ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_002958 --> ing2_Yeast_extract__BD_Difco_
    ing3_Agar__if_needed_["Agar (if needed)"]
    CultureMech_002958 --> ing3_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_needed_ -.-> CHEBI_2509
    ing4_Distilled_water["Distilled water"]
    CultureMech_002958 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Tryptone (BD-Difco) 0.5 G_PER_L MICRO:0000182 (source link unavailable)
Tryptone
 MediaDive J610 / JCM Medium 610 lists 0.5 g/L Tryptone (BD-Difco).
2 Glycerol
C3H8O3 92.094 g/mol
1.0 G_PER_L CHEBI:17754
glycerol
 MediaDive J610 / JCM Medium 610 lists 1.0 g/L Glycerol.
3 Yeast extract (BD-Difco) 0.1 G_PER_L FOODON:03315426
yeast extract
 MediaDive J610 / JCM Medium 610 lists 0.1 g/L Yeast extract (BD-Difco).
4 Agar (if needed) 10.0 G_PER_L CHEBI:2509
agar
 MediaDive J610 / JCM Medium 610 lists 10 g/L Agar if needed.
5 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 MediaDive J610 / JCM Medium 610 lists 1.0 L Distilled water.

Preparation

  1. Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.084391Z mediadive-import Imported from MediaDive Source: JCM, ID: J610
2026-03-13T06:19:16.823774Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002958
2026-03-17T06:30:38.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.431662+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SPHAEROTILUS MEDIUM" to "sphaerotilus_medium" for consistent matching
2026-06-05T07:40:17.516820+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m618_score15.py RESOLVED_JCM_610_SPHAEROTILUS Restored the JCM Medium 610 distilled-water component omitted by the MediaDive import, grounded tryptone and yeast extract, added JCM autoclave metadata, and linked the TOGO M618 source duplicate.