desulfomicrobium_medium

CultureMech:002963 bacterial DEFINED LIQUID
Original name: DESULFOMICROBIUM MEDIUM

Overview

External ontology
mediadive.medium:J616 (source link unavailable) — DESULFOMICROBIUM MEDIUM
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=616

Composition

flowchart LR
    CultureMech_002963["desulfomicrobium_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002963 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002963 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002963 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002963 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_KCl["KCl"]
    CultureMech_002963 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Na2SO4["Na2SO4"]
    CultureMech_002963 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_Sodium_lactate["Sodium lactate"]
    CultureMech_002963 --> ing6_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing6_Sodium_lactate -.-> CHEBI_75228
    ing7_NaHCO3["NaHCO3"]
    CultureMech_002963 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002963 --> ing8_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing8_Na2S_x_9_H2O -.-> CHEBI_76209
    ing9_Na2_EDTA["Na2-EDTA"]
    CultureMech_002963 --> ing9_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing9_Na2_EDTA -.-> CHEBI_64734
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002963 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_MnCl4_x_4_H2O["MnCl4 x 4 H2O"]
    CultureMech_002963 --> ing11_MnCl4_x_4_H2O
    ing12_ZnCl2["ZnCl2"]
    CultureMech_002963 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002963 --> ing13_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002963 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_H3BO3["H3BO3"]
    CultureMech_002963 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002963 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002963 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing18_Biotin["Biotin"]
    CultureMech_002963 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_002963 --> ing19_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing19_DL_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (20)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.242014 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.484027 G_PER_L CHEBI:31206
ammonium chloride
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0484027 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.484027 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 KCl
Cl.K 74.551 g/mol
0.484027 G_PER_L CHEBI:32588
potassium chloride
6 Na2SO4
2Na.O4S 142.043 g/mol
1.37464 G_PER_L CHEBI:32149
sodium sulfate
7 Sodium lactate
C3H5O3.Na 112.06 g/mol
1.08422 G_PER_L CHEBI:75228
sodium lactate
8 NaHCO3
CHO3.Na 84.006 g/mol
30 G_PER_L CHEBI:32139
sodium hydrogencarbonate
9 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
1 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
10 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
3 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 MnCl4 x 4 H2O 0.05 G_PER_L
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.042 G_PER_L CHEBI:49976
zinc dichloride
14 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.018 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.08 G_PER_L CHEBI:30753
4-aminobenzoic acid
19 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
20 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Preparation

  1. Mix ingredients and autoclave. Cool under a N2-CO2 gas mixture (4:1, v/v) and add the following solutions from sterile stocks:
  2. Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Adjust pH to 7.0 - 7.2, if necessary.

Solutions

Trace element solution SL12B

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1.1 G_PER_L

Vitamin solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Vitamin B12 0.1 G_PER_L
Nicotinic acid 0.2 G_PER_L
Pyridoxine hydrochloride 0.3 G_PER_L
Thiamine HCl 0.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.095355Z mediadive-import Imported from MediaDive Source: JCM, ID: J616
2026-03-13T06:19:16.847763Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002963
2026-03-17T06:30:01.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.459141+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOMICROBIUM MEDIUM" to "desulfomicrobium_medium" for consistent matching
2026-06-05T07:40:56.800016+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:55.652095+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T23:31:54.379152+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace element solution SL12B' @ 1 ml/l; 4 -> 'Vitamin solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).