Overview
- External ontology
- mediadive.medium:J618 (source link unavailable) — CYS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- JCM Medium 618 CYS Medium lists 3 g N-Z-Case, 2 g yeast extract, 1 g soluble starch, 20 g NaCl, 0.125 g MgCl2 x 6 H2O, 0.025 g CaCl2 x 2 H2O, 0.01 g FeSO4 x 7 H2O, and seven trace-metal stocks added at 100 uL each before pH adjustment to 7.5 and final volume to 1 L.
Composition
flowchart LR
CultureMech_002965["cys_medium"]
ing0_N_Z_Case__Wako_["N-Z-Case (Wako)"]
CultureMech_002965 --> ing0_N_Z_Case__Wako_
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_002965 --> ing1_Yeast_extract__BD_Difco_
ing2_Soluble_starch__BD_Difco_["Soluble starch (BD-Difco)"]
CultureMech_002965 --> ing2_Soluble_starch__BD_Difco_
CHEBI_28017["CHEBI:28017"]
ing2_Soluble_starch__BD_Difco_ -.-> CHEBI_28017
ing3_NaCl["NaCl"]
CultureMech_002965 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002965 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002965 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002965 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_Distilled_water["Distilled water"]
CultureMech_002965 --> ing7_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing7_Distilled_water -.-> CHEBI_15377
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | N-Z-Case (Wako) | 3.0 G_PER_L | MediaDive J618 / JCM Medium 618 lists 3.0 g/L N-Z-Case (Wako). | |
| 2 | Yeast extract (BD-Difco) | 2.0 G_PER_L |
FOODON:03315426
yeast extract
|
MediaDive J618 / JCM Medium 618 lists 2.0 g/L Yeast extract (BD-Difco). |
| 3 | Soluble starch (BD-Difco) | 1.0 G_PER_L |
CHEBI:28017
starch
|
MediaDive J618 / JCM Medium 618 lists 1.0 g/L Soluble starch (BD-Difco). |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
20.0 G_PER_L |
CHEBI:26710
sodium chloride
|
MediaDive J618 / JCM Medium 618 lists 20.0 g/L NaCl. |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.125 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
MediaDive J618 / JCM Medium 618 lists 0.125 g/L MgCl2 x 6 H2O. |
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.025 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
MediaDive J618 / JCM Medium 618 lists 0.025 g/L CaCl2 x 2 H2O. |
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
MediaDive J618 / JCM Medium 618 lists 0.01 g/L FeSO4 x 7 H2O. |
| 8 |
Distilled water
H2O 18.015 g/mol
|
900.0 ML_PER_L |
CHEBI:15377
water
|
MediaDive J618 / JCM Medium 618 lists 900 ml distilled water before pH adjustment. |
Preparation
- Add the base components to 900 ml distilled water and mix thoroughly.
- Add each trace-metal stock solution at 0.1 ml/L in the listed order.
- Adjust pH to 7.5, bring the volume to 1.0 L with distilled water, and autoclave.
- Inoculate into the medium prewarmed to the optimal growth temperature.
Solutions
Na2MoO4 x 2 H2O stock
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2MoO4 x 2 H2O | 12.0 G_PER_L | Na2MoO4 x 2 H2O stock is represented as a 12.0 g/L Na2MoO4 x 2 H2O stock. |
MediaDive J618 / JCM Medium 618 adds 100 uL of 12.0 g/L Na2MoO4 x 2 H2O.
VOSO4 x n H2O stock
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| VOSO4 x n H2O | 1.0 G_PER_L | VOSO4 x n H2O stock is represented as a 1.0 g/L VOSO4 x n H2O stock. |
MediaDive J618 / JCM Medium 618 adds 100 uL of 1.0 g/L VOSO4 x n H2O.
MnCl2 x 4 H2O stock
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MnCl2 x 4 H2O | 5.0 G_PER_L | MnCl2 x 4 H2O stock is represented as a 5.0 g/L MnCl2 x 4 H2O stock in 0.01 N H2SO4. |
MediaDive J618 / JCM Medium 618 adds 100 uL of 5.0 g/L MnCl2 x 4 H2O in 0.01 N H2SO4.
ZnSO4 x 7 H2O stock
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| ZnSO4 x 7 H2O | 0.6 G_PER_L | ZnSO4 x 7 H2O stock is represented as a 0.6 g/L ZnSO4 x 7 H2O stock in 0.01 N H2SO4. |
MediaDive J618 / JCM Medium 618 adds 100 uL of 0.6 g/L ZnSO4 x 7 H2O in 0.01 N H2SO4.
CuSO4 x 5 H2O stock
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CuSO4 x 5 H2O | 0.15 G_PER_L | CuSO4 x 5 H2O stock is represented as a 0.15 g/L CuSO4 x 5 H2O stock in 0.01 N H2SO4. |
MediaDive J618 / JCM Medium 618 adds 100 uL of 0.15 g/L CuSO4 x 5 H2O in 0.01 N H2SO4.
CoCl2 x 6 H2O stock
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| CoCl2 x 6 H2O | 8.0 G_PER_L | CoCl2 x 6 H2O stock is represented as a 8.0 g/L CoCl2 x 6 H2O stock in 0.01 N H2SO4. |
MediaDive J618 / JCM Medium 618 adds 100 uL of 8.0 g/L CoCl2 x 6 H2O in 0.01 N H2SO4.
NiCl2 x 6 H2O stock
Dose: 0.1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NiCl2 x 6 H2O | 0.2 G_PER_L | NiCl2 x 6 H2O stock is represented as a 0.2 g/L NiCl2 x 6 H2O stock in 0.01 N H2SO4. |
MediaDive J618 / JCM Medium 618 adds 100 uL of 0.2 g/L NiCl2 x 6 H2O in 0.01 N H2SO4.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.102180Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J618 |
| 2026-03-13T06:19:16.859100Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002965 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.471476+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CYS MEDIUM" to "cys_medium" for consistent matching |
| 2026-06-05T07:40:17.529917+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m627_m628_score15.py | RESOLVED_JCM_618_CYS | Corrected the MediaDive import's 900 ml base scaling, represented the seven 100 uL trace-metal additions as stock solutions, added their CHEBI groundings, retained N-Z-Case as an intentionally unmapped Wako product, and linked JCM 619 and TOGO M627. |