Overview
- External ontology
- mediadive.medium:J621 (source link unavailable) — HYL MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=621
Composition
flowchart LR
CultureMech_002968["hyl_medium"]
ing0_HEPES["HEPES"]
CultureMech_002968 --> ing0_HEPES
CHEBI_46756["CHEBI:46756"]
ing0_HEPES -.-> CHEBI_46756
ing1_NaCl["NaCl"]
CultureMech_002968 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Na2SO4["Na2SO4"]
CultureMech_002968 --> ing2_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing2_Na2SO4 -.-> CHEBI_32149
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002968 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_NH4Cl["NH4Cl"]
CultureMech_002968 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KCl["KCl"]
CultureMech_002968 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002968 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_K2HPO4["K2HPO4"]
CultureMech_002968 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_Yeast_extract["Yeast extract"]
CultureMech_002968 --> ing8_Yeast_extract
ing9_Sodium_lactate["Sodium lactate"]
CultureMech_002968 --> ing9_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing9_Sodium_lactate -.-> CHEBI_75228
ing10_Resazurin["Resazurin"]
CultureMech_002968 --> ing10_Resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Resazurin -.-> CHEBI_8806
ing11_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002968 --> ing11_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing11_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002968 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_Na2_EDTA["Na2-EDTA"]
CultureMech_002968 --> ing13_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing13_Na2_EDTA -.-> CHEBI_64734
ing14_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
CultureMech_002968 --> ing14_NiSO4_x_6_H2O
CHEBI_53001["CHEBI:53001"]
ing14_NiSO4_x_6_H2O -.-> CHEBI_53001
ing15_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002968 --> ing15_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing15_CuSO4_x_5_H2O -.-> CHEBI_31440
ing16_H3BO3["H3BO3"]
CultureMech_002968 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_002968 --> ing17_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing17_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002968 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002968 --> ing19_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing20_Na2SeO3["Na2SeO3"]
CultureMech_002968 --> ing20_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing20_Na2SeO3 -.-> CHEBI_48843
ing21_Biotin["Biotin"]
CultureMech_002968 --> ing21_Biotin
CHEBI_15956["CHEBI:15956"]
ing21_Biotin -.-> CHEBI_15956
ing22_Folic_acid["Folic acid"]
CultureMech_002968 --> ing22_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing22_Folic_acid -.-> CHEBI_27470
ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002968 --> ing23_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing24_Thiamine_HCl["Thiamine HCl"]
CultureMech_002968 --> ing24_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing24_Thiamine_HCl -.-> CHEBI_49105
ing25_Riboflavin["Riboflavin"]
CultureMech_002968 --> ing25_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing25_Riboflavin -.-> CHEBI_17015
ing26_Nicotinic_acid["Nicotinic acid"]
CultureMech_002968 --> ing26_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing26_Nicotinic_acid -.-> CHEBI_15940
ing27_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002968 --> ing27_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing27_Calcium_pantothenate -.-> CHEBI_31345
ing28_Vitamin_B12["Vitamin B12"]
CultureMech_002968 --> ing28_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing28_Vitamin_B12 -.-> CHEBI_176843
ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002968 --> ing29_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
CultureMech_002968 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | HEPES | 4.66667 G_PER_L |
CHEBI:46756
HEPES
|
|
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
19.6078 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.92157 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.21569 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.245098 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.333333 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.137255 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.137255 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 | Yeast extract | 0.980392 G_PER_L | ||
| 10 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
4.5 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 11 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000980392 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
1 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
4 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
29 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 15 |
NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
|
0.11 G_PER_L |
CHEBI:53001
nickel sulfate
|
|
| 16 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.1 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 21 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.05 G_PER_L |
CHEBI:48843
disodium selenite
|
|
| 22 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 23 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 24 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 25 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 26 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 27 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 28 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 29 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 30 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 31 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Adjust pH to 7.0.
- Autoclave and cool down to room temperature while flushing with N2. Then add the following solutions from sterile anaerobic stocks:
- First dissolve EDTA x 2Na and adjust pH to 8.0 with KOH. Then add the minerals.
Solutions
Trace elements
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MnSO4 x n H2O | 3.26 G_PER_L | |
| CoCl2 x 6 H2O | 1.8 G_PER_L | |
| ZnSO4 x 7 H2O | 1 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.109282Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J621 |
| 2026-01-28T19:48:07.936770Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:16.878719Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002968 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.493110+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HYL MEDIUM" to "hyl_medium" for consistent matching |
| 2026-06-05T07:41:09.156598+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.969902+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-13T23:31:54.397680+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace elements' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |