Overview
- External ontology
- mediadive.medium:J631 (source link unavailable) — MMB MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=631
Composition
flowchart LR
CultureMech_002978["mmb_medium"]
ing0_Peptone["Peptone"]
CultureMech_002978 --> ing0_Peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002978 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_002978 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_002978 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002978 --> ing4_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing4_Nitrilotriacetic_acid -.-> CHEBI_44557
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002978 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002978 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7__NH4_6Mo7O24_x_4_H2O["(NH4)6Mo7O24 x 4 H2O"]
CultureMech_002978 --> ing7__NH4_6Mo7O24_x_4_H2O
CHEBI_91249["CHEBI:91249"]
ing7__NH4_6Mo7O24_x_4_H2O -.-> CHEBI_91249
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002978 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_Nicotinic_acid["Nicotinic acid"]
CultureMech_002978 --> ing9_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing9_Nicotinic_acid -.-> CHEBI_15940
ing10_Thiamine_HCl["Thiamine HCl"]
CultureMech_002978 --> ing10_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing10_Thiamine_HCl -.-> CHEBI_49105
ing11_Biotin["Biotin"]
CultureMech_002978 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_002978 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002978 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Riboflavin["Riboflavin"]
CultureMech_002978 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002978 --> ing15_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing15_Calcium_pantothenate -.-> CHEBI_31345
ing16_Vitamin_B12["Vitamin B12"]
CultureMech_002978 --> ing16_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing16_Vitamin_B12 -.-> CHEBI_176843
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002978 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18_Lipoic_acid["Lipoic acid"]
CultureMech_002978 --> ing18_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing18_Lipoic_acid -.-> CHEBI_16494
ing19_Na2_EDTA["Na2-EDTA"]
CultureMech_002978 --> ing19_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing19_Na2_EDTA -.-> CHEBI_64734
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002978 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002978 --> ing21_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing21_MnSO4_x_n_H2O -.-> CHEBI_86360
ing22_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002978 --> ing22_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing22_CuSO4_x_5_H2O -.-> CHEBI_31440
ing23_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_002978 --> ing23_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing23_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
ing24_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_002978 --> ing24_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing24_Na2B4O7_x_10_H2O -.-> CHEBI_131366
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 0.0970874 G_PER_L | ||
| 2 | Yeast extract | 0.0970874 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
0.970874 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.194175 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
10 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
14.45 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
4.42 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
(NH4)6Mo7O24 x 4 H2O
2H4N.MoO4 196.014 g/mol
|
0.00925 G_PER_L |
CHEBI:91249
ammonium molybdate
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.599 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.099, 0.5] |
| 10 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.055 G_PER_L |
CHEBI:15940
nicotinic acid
|
[Merged 2 duplicates: 0.05, 0.005] |
| 11 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.030000000000000002 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
[Merged 2 duplicates: 0.025, 0.005] |
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0025 G_PER_L |
CHEBI:15956
biotin
|
[Merged 2 duplicates: 0.0005, 0.002] |
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 16 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 17 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 19 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 20 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.25 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.095 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 22 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.154 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 23 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.0392 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 24 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.0248 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
|
| 25 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0177 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Preparation
- Adjust pH to 7.0 - 7.5.
- For preparation of solid medium, add 15.0 g/L agar.
- Dissolve and neutralized nitrilotriacetic acid in 500.0 ml distilled water with the addition of KOH. Add the remaining chemicals in order listed. Adjust pH to 6.8 and bring volume to 1.0 L with distilled water.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.136049Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J631 |
| 2026-03-13T03:08:39.064655Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:16.932072Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002978 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.551527+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMB MEDIUM" to "mmb_medium" for consistent matching |
| 2026-06-05T07:40:17.542542+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.051724+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |