clostridium_bovis_medium

Overview

External ontology

mediadive.medium:J635 (source link unavailable) — CLOSTRIDIUM BOVIS MEDIUM

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=635 Composition recovery source: https://togomedium.org/medium/M649 (TOGO snapshot of JCM medium J635).

Composition

flowchart LR
    CultureMech_002982["clostridium_bovis_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_002982 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_002982 --> ing1_MgSO4_7H2O
    ing2_yeast_extract["yeast extract"]
    CultureMech_002982 --> ing2_yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_002982 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_002982 --> ing4_CaCl2_2H2O
    ing5_KH2PO4["KH2PO4"]
    CultureMech_002982 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_K2HPO4["K2HPO4"]
    CultureMech_002982 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_Resazurin["Resazurin"]
    CultureMech_002982 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_002982 --> ing8_Na2S_9H2O
    ing9__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002982 --> ing9__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing9__NH4_2SO4 -.-> CHEBI_62946
    ing10_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
    CultureMech_002982 --> ing10_L__Cysteine_HCl_H2O
    ing11_peptone["peptone"]
    CultureMech_002982 --> ing11_peptone
    ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_002982 --> ing12_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing12_Carbon_dioxide_gas -.-> CHEBI_16526
    ing13_Nitrogen_gas["Nitrogen gas"]
    CultureMech_002982 --> ing13_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing13_Nitrogen_gas -.-> CHEBI_17997

Ingredients (14)

Preparation

1. Mix components except L--cysteine・HCl・H2O and Na2S・9H2O and adjust pH to 7.2. Bring to a boil for 5--10 sec and cool down under a stream of N2--CO2 (80:20, v/v). Distribute into culture vessels under the same gasmixture, seal with butyl rubber stoppers and autoclave. Separately autoclave L--cysteine・HCl・H2O and Na2S・9H2O as 5% solutions under a N2 atmosphere. Prior to inoculation, add L--cysteine・HCl・H2O and Na2S・9H2O solutions anaerobically.
2. Use Medium No. [634] supplemented with 0.5 g/L (final conc.) yeast extract and 0.2 g/L peptone instead of sodium succinate.

Target organisms

No target organisms are recorded.

Provenance