sautons_synthetic_medium (CultureMech:002986)

Overview

External ontology

mediadive.medium:J63 (source link unavailable) — SAUTON'S SYNTHETIC MEDIUM

Applications

Microbial cultivation

pH

7.2

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=63

Composition

flowchart LR
    CultureMech_002986["sautons_synthetic_medium"]
    ing0_L_Asparagine["L-Asparagine"]
    CultureMech_002986 --> ing0_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing0_L_Asparagine -.-> CHEBI_17196
    ing1_Citric_acid["Citric acid"]
    CultureMech_002986 --> ing1_Citric_acid
    CHEBI_30769["CHEBI:30769"]
    ing1_Citric_acid -.-> CHEBI_30769
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002986 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002986 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_002986 --> ing4_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing4_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing5_Glycerol["Glycerol"]
    CultureMech_002986 --> ing5_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing5_Glycerol -.-> CHEBI_17754
    ing6_Agar["Agar"]
    CultureMech_002986 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

#	Ingredient	Concentration	Ontology
1	L-Asparagine C4H8N2O3 132.119 g/mol	3.77358 G_PER_L	CHEBI:17196 L-asparagine
2	Citric acid C6H8O7 192.123 g/mol	1.88679 G_PER_L	CHEBI:30769 citric acid
3	K2HPO4 HO4P.2K 174.174 g/mol	0.471698 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.471698 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
5	Ferric ammonium citrate	0.0471698 G_PER_L	CHEBI:31604 ferric ammonium citrate
6	Glycerol C3H8O3 92.094 g/mol	60 G_PER_L	CHEBI:17754 glycerol
7	Agar	14.1509 G_PER_L	CHEBI:2509 agar

Preparation

Adjust pH to 7.2 with NH4OH.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-26T17:53:15.667996Z	mediadive-import	Imported from MediaDive	Source: JCM, ID: J63
2026-03-13T06:19:16.976846Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:002986
2026-03-17T06:30:40.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.602372+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "SAUTON'S SYNTHETIC MEDIUM" to "sautons_synthetic_medium" for consistent matching
2026-06-05T07:41:45.305930+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.316803+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T14:33:27.670564+00:00	codex	CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged.