Overview
- External ontology
- mediadive.medium:J646 (source link unavailable) — RHODOSPIRILLUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=646
Composition
flowchart LR
CultureMech_002992["rhodospirillum_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002992 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002992 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_002992 --> ing2_Yeast_extract
ing3_Peptone["Peptone"]
CultureMech_002992 --> ing3_Peptone
ing4_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002992 --> ing4_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing4_Sodium_pyruvate -.-> CHEBI_50144
ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002992 --> ing5_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing5_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 | Yeast extract | 10 G_PER_L | ||
| 4 | Peptone | 10 G_PER_L | ||
| 5 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 6 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.120091 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Adjust pH to 7.0.
- After autoclaving, add filter-sterilized Na2S x 9H2O solution to the medium.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.177396Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J646 |
| 2026-03-13T06:19:17.021924Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002992 |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.655533+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RHODOSPIRILLUM MEDIUM" to "rhodospirillum_medium" for consistent matching |
| 2026-06-05T07:41:44.426606+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.997122+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |