rhodovulum_kholense_medium

CultureMech:002995 bacterial COMPLEX LIQUID
Original name: RHODOVULUM KHOLENSE MEDIUM

Overview

External ontology
mediadive.medium:J649 (source link unavailable) — RHODOVULUM KHOLENSE MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=649

Composition

flowchart LR
    CultureMech_002995["rhodovulum_kholense_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002995 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002995 --> ing1_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002995 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002995 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_NaCl["NaCl"]
    CultureMech_002995 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_002995 --> ing5_Yeast_extract
    ing6_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002995 --> ing6_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Sodium_pyruvate -.-> CHEBI_50144

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.499501 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.11988 G_PER_L CHEBI:86158
calcium chloride dihydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1.998 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.639361 G_PER_L CHEBI:31206
ammonium chloride
5 NaCl
Cl.Na 58.443 g/mol
19.98 G_PER_L CHEBI:26710
sodium chloride
6 Yeast extract 0.499501 G_PER_L
7 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
2.997 G_PER_L CHEBI:50144
sodium pyruvate

Preparation

  1. Adjust pH to 7.0.

Solutions

micronutrient solution

Dose: 1 G_PER_L

See mediadive_6236_Micronutrient_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.186433Z mediadive-import Imported from MediaDive Source: JCM, ID: J649
2026-03-09T08:04:21.179306+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:17.042949Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002995
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.680677+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "RHODOVULUM KHOLENSE MEDIUM" to "rhodovulum_kholense_medium" for consistent matching
2026-05-16T09:02:59.259014+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:17.551994+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.056867+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.