Overview
- External ontology
- mediadive.medium:J651 (source link unavailable) — SAP2 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=651
Composition
flowchart LR
CultureMech_002997["sap2_medium"]
ing0_Tryptone["Tryptone"]
CultureMech_002997 --> ing0_Tryptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002997 --> ing1_Yeast_extract
ing2_Agar["Agar"]
CultureMech_002997 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_NaCl["NaCl"]
CultureMech_002997 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KCl["KCl"]
CultureMech_002997 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002997 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002997 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002997 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 1 G_PER_L | ||
| 2 | Yeast extract | 1 G_PER_L | ||
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.35 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.7 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.192771Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J651 |
| 2026-03-13T06:19:17.056537Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002997 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.696604+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SAP2 MEDIUM" to "sap2_medium" for consistent matching |
| 2026-06-05T07:41:45.284330+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |