Overview
- External ontology
- mediadive.medium:J659 (source link unavailable) — PELAGICOCCUS AGAR
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- JCM Medium 659 Pelagicoccus Agar lists 55.1 g Marine agar 2216 (BD-Difco), 9.1 g R2A agar (BD-Difco), 750.0 ml Artificial seawater, and 250.0 ml Distilled water, then adjusts pH to 7.5.
Composition
flowchart LR
CultureMech_003004["pelagicoccus_agar"]
ing0_Marine_agar_2216__BD_Difco_["Marine agar 2216 (BD-Difco)"]
CultureMech_003004 --> ing0_Marine_agar_2216__BD_Difco_
ing1_R2A_agar__BD_Difco_["R2A agar (BD-Difco)"]
CultureMech_003004 --> ing1_R2A_agar__BD_Difco_
ing2_Distilled_water["Distilled water"]
CultureMech_003004 --> ing2_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing2_Distilled_water -.-> CHEBI_15377
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Marine agar 2216 (BD-Difco) | 55.1 G_PER_L | MediaDive J659 / JCM Medium 659 lists 55.1 g/L Marine agar 2216 (BD-Difco); this commercial medium product is retained as an opaque complex component. | |
| 2 | R2A agar (BD-Difco) | 9.1 G_PER_L | MediaDive J659 / JCM Medium 659 lists 9.1 g/L R2A agar (BD-Difco); this commercial medium product is retained as an opaque complex component. | |
| 3 |
Distilled water
H2O 18.015 g/mol
|
250.0 ML_PER_L |
CHEBI:15377
water
|
MediaDive J659 / JCM Medium 659 lists 250.0 ml/L Distilled water. |
Preparation
- Combine the listed commercial medium components with 750.0 ml Artificial seawater and 250.0 ml distilled water.
- Adjust pH to 7.5.
Solutions
Artificial seawater
Dose: 750.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaCl | 24.0 G_PER_L | JCM Medium 659 lists 24.0 g/L NaCl. |
| MgSO4 x 7 H2O | 7.0 G_PER_L | JCM Medium 659 lists 7.0 g/L MgSO4 x 7 H2O. |
| MgCl2 x 6 H2O | 5.3 G_PER_L | JCM Medium 659 lists 5.3 g/L MgCl2 x 6 H2O. |
| KCl | 0.7 G_PER_L | JCM Medium 659 lists 0.7 g/L KCl. |
| CaCl2 x 2 H2O | 0.1 G_PER_L | JCM Medium 659 lists 0.1 g/L CaCl2 x 2 H2O. |
| Distilled water | 1.0 L | JCM Medium 659 lists 1.0 L Distilled water in the Artificial seawater stock solution. |
JCM Medium 659 adds 750.0 ml/L Artificial seawater prepared from the printed subrecipe.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.210089Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J659 |
| 2026-03-13T06:19:17.090753Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003004 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.737568+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PELAGICOCCUS AGAR" to "pelagicoccus_agar" for consistent matching |
| 2026-06-05T07:41:41.126706+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m675_m676_score15.py | RESOLVED_JCM_659_PELAGICOCCUS_AGAR | Restored JCM Medium 659 as the printed agar recipe, represented Artificial seawater as a 750.0 ml/L stock solution instead of flattening its salt recipe into the medium, de-grounded the commercial Marine agar and R2A agar products, and linked TOGO M675 and M676. |