Overview
- External ontology
- mediadive.medium:J65 (source link unavailable) — CELLULOSE AGAR
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=65
Composition
flowchart LR
CultureMech_003005["cellulose_agar"]
ing0_NH4NO3["NH4NO3"]
CultureMech_003005 --> ing0_NH4NO3
CHEBI_63038["CHEBI:63038"]
ing0_NH4NO3 -.-> CHEBI_63038
ing1_Urea["Urea"]
CultureMech_003005 --> ing1_Urea
CHEBI_16199["CHEBI:16199"]
ing1_Urea -.-> CHEBI_16199
ing2_Casein["Casein"]
CultureMech_003005 --> ing2_Casein
ing3_L_Asparagine["L-Asparagine"]
CultureMech_003005 --> ing3_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing3_L_Asparagine -.-> CHEBI_17196
ing4_K2HPO4["K2HPO4"]
CultureMech_003005 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003005 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaCO3["CaCO3"]
CultureMech_003005 --> ing6_CaCO3
CHEBI_3311["CHEBI:3311"]
ing6_CaCO3 -.-> CHEBI_3311
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003005 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_Yeast_extract["Yeast extract"]
CultureMech_003005 --> ing8_Yeast_extract
ing9_Casamino_acids["Casamino acids"]
CultureMech_003005 --> ing9_Casamino_acids
ing10_Cellulose["Cellulose"]
CultureMech_003005 --> ing10_Cellulose
CHEBI_18246["CHEBI:18246"]
ing10_Cellulose -.-> CHEBI_18246
ing11_Agar["Agar"]
CultureMech_003005 --> ing11_Agar
CHEBI_2509["CHEBI:2509"]
ing11_Agar -.-> CHEBI_2509
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4NO3
H4N.NO3 80.043 g/mol
|
0.2 G_PER_L |
CHEBI:63038
ammonium nitrate
|
|
| 2 |
Urea
CH4N2O 60.056 g/mol
|
0.1 G_PER_L |
CHEBI:16199
urea
|
|
| 3 | Casein | 0.2 G_PER_L | ||
| 4 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
0.25 G_PER_L |
CHEBI:17196
L-asparagine
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
CaCO3
CO3.Ca 100.086 g/mol
|
0.2 G_PER_L |
CHEBI:3311
calcium carbonate
|
|
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.005 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 9 | Yeast extract | 0.1 G_PER_L | ||
| 10 | Casamino acids | 0.1 G_PER_L | ||
| 11 |
Cellulose
(C6H10O5)n.H2O 180.156 g/mol
|
20 G_PER_L |
CHEBI:18246
(1->4)-beta-D-glucan
|
|
| 12 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- pH unadjusted.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:15.672290Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J65 |
| 2026-01-28T19:48:26.498751Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:17.096475Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003005 |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.743839+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CELLULOSE AGAR" to "cellulose_agar" for consistent matching |
| 2026-06-05T07:40:52.943574+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:36.281287+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:54.422495+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T14:33:27.670564+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |