flexibacter_canadensis_medium

CultureMech:003017 bacterial COMPLEX LIQUID
Original name: FLEXIBACTER CANADENSIS MEDIUM

Overview

External ontology
mediadive.medium:J672 (source link unavailable) — FLEXIBACTER CANADENSIS MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=672

Composition

flowchart LR
    CultureMech_003017["flexibacter_canadensis_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003017 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_KNO3["KNO3"]
    CultureMech_003017 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003017 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Sodium_glycerophosphate["Sodium glycerophosphate"]
    CultureMech_003017 --> ing3_Sodium_glycerophosphate
    ing4_Tris_hydroxymethyl_aminomethane["Tris(hydroxymethyl)aminomethane"]
    CultureMech_003017 --> ing4_Tris_hydroxymethyl_aminomethane
    CHEBI_9754["CHEBI:9754"]
    ing4_Tris_hydroxymethyl_aminomethane -.-> CHEBI_9754
    ing5_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003017 --> ing5_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing5_Thiamine_HCl -.-> CHEBI_49105
    ing6_Casamino_acids["Casamino acids"]
    CultureMech_003017 --> ing6_Casamino_acids
    ing7_Glucose["Glucose"]
    CultureMech_003017 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_Vitamin_B12["Vitamin B12"]
    CultureMech_003017 --> ing8_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing8_Vitamin_B12 -.-> CHEBI_176843
    ing9_HCl["HCl"]
    CultureMech_003017 --> ing9_HCl
    CHEBI_17883["CHEBI:17883"]
    ing9_HCl -.-> CHEBI_17883
    ing10_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003017 --> ing10_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing10_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing11_ZnCl2["ZnCl2"]
    CultureMech_003017 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003017 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_H3BO3["H3BO3"]
    CultureMech_003017 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003017 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003017 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003017 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003017 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0999001 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 KNO3
K.NO3 101.102 g/mol
0.0999001 G_PER_L CHEBI:63043
potassium nitrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0999001 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 Sodium glycerophosphate 0.0999001 G_PER_L
5 Tris(hydroxymethyl)aminomethane
C4H11NO3 121.136 g/mol
0.999001 G_PER_L CHEBI:9754
tris
6 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.000999001 G_PER_L CHEBI:49105
thiamine hydrochloride
7 Casamino acids 0.999001 G_PER_L
8 Glucose
C6H12O6 180.156 g/mol
0.999001 G_PER_L CHEBI:17234
glucose
9 Vitamin B12 1 G_PER_L CHEBI:176843
vitamin B12
10 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
11 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 7.5.
  2. Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.246486Z mediadive-import Imported from MediaDive Source: JCM, ID: J672
2026-01-28T19:48:19.494050Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:17.186243Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003017
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.839028+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "FLEXIBACTER CANADENSIS MEDIUM" to "flexibacter_canadensis_medium" for consistent matching
2026-06-05T07:40:17.561426+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.383011+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:37:34.104121+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:43.059717+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.