methanopyrus_medium_for_strain_116

CultureMech:003018 archaea DEFINED LIQUID
Original name: METHANOPYRUS MEDIUM FOR STRAIN 116

Overview

External ontology
mediadive.medium:J673 (source link unavailable) — METHANOPYRUS MEDIUM FOR STRAIN 116
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=673

Composition

flowchart LR
    CultureMech_003018["methanopyrus_medium_for_strain_116"]
    ing0_NaCl["NaCl"]
    CultureMech_003018 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003018 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_KH2PO4["KH2PO4"]
    CultureMech_003018 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003018 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_003018 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003018 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003018 --> ing6_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_003018 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003018 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003018 --> ing9_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing9_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing10_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
    CultureMech_003018 --> ing10_Fe2_SO4_3_x_n_H2O
    CHEBI_53438["CHEBI:53438"]
    ing10_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
    ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003018 --> ing11_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing12_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003018 --> ing12_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing12_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing13_Resazurin["Resazurin"]
    CultureMech_003018 --> ing13_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing13_Resazurin -.-> CHEBI_8806
    ing14_Iron["Iron"]
    CultureMech_003018 --> ing14_Iron
    CHEBI_18248["CHEBI:18248"]
    ing14_Iron -.-> CHEBI_18248
    ing15_NaHCO3["NaHCO3"]
    CultureMech_003018 --> ing15_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing15_NaHCO3 -.-> CHEBI_32139
    ing16_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003018 --> ing16_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing16_Na2S_x_9_H2O -.-> CHEBI_76209
    ing17_NaBr["NaBr"]
    CultureMech_003018 --> ing17_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing17_NaBr -.-> CHEBI_63004
    ing18_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_003018 --> ing18_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing18_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing19_H3BO3["H3BO3"]
    CultureMech_003018 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_KI["KI"]
    CultureMech_003018 --> ing20_KI
    CHEBI_8346["CHEBI:8346"]
    ing20_KI -.-> CHEBI_8346
    ing21_Sodium_silicate["Sodium silicate"]
    CultureMech_003018 --> ing21_Sodium_silicate
    CHEBI_60720["CHEBI:60720"]
    ing21_Sodium_silicate -.-> CHEBI_60720
    ing22_NaF["NaF"]
    CultureMech_003018 --> ing22_NaF
    CHEBI_28741["CHEBI:28741"]
    ing22_NaF -.-> CHEBI_28741
    ing23_KNO3["KNO3"]
    CultureMech_003018 --> ing23_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing23_KNO3 -.-> CHEBI_63043
    ing24_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_003018 --> ing24_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing24_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing25_Biotin["Biotin"]
    CultureMech_003018 --> ing25_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing25_Biotin -.-> CHEBI_15956
    ing26_Folic_acid["Folic acid"]
    CultureMech_003018 --> ing26_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing26_Folic_acid -.-> CHEBI_27470
    ing27_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003018 --> ing27_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing27_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing28_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003018 --> ing28_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing28_Thiamine_HCl -.-> CHEBI_49105
    ing29_Riboflavin["Riboflavin"]
    CultureMech_003018 --> ing29_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing29_Riboflavin -.-> CHEBI_17015
    CultureMech_003018 --> more["...12 more"]

Ingredients (42)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
19.6078 G_PER_L CHEBI:26710
sodium chloride
2 K2HPO4
HO4P.2K 174.174 g/mol
0.0686275 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 KH2PO4
H2O4P.K 136.084 g/mol
0.0882353 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.784314 G_PER_L CHEBI:86158
calcium chloride dihydrate
5 NH4Cl
Cl.H4N 53.492 g/mol
0.294118 G_PER_L CHEBI:31206
ammonium chloride
6 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
5.88235 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
7 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.980392 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
8 KCl
Cl.K 74.551 g/mol
0.323529 G_PER_L CHEBI:32588
potassium chloride
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.00490196 G_PER_L CHEBI:34887
nickel dichloride
10 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.00490196 G_PER_L CHEBI:131361
disodium selenite pentahydrate
11 Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
0.0490196 G_PER_L CHEBI:53438
iron(3+) sulfate
12 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.00980392 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
13 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.00490196 G_PER_L CHEBI:63939
sodium tungstate dihydrate
14 Resazurin
C12H7NO4 229.191 g/mol
0.000980392 G_PER_L CHEBI:8806
Resazurin
15 Iron
Fe 55.845 g/mol
19.6078 G_PER_L CHEBI:18248
iron atom
16 NaHCO3
CHO3.Na 84.006 g/mol
1.96078 G_PER_L CHEBI:32139
sodium hydrogencarbonate
17 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.490196 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
18 NaBr
Br.Na 102.894 g/mol
4.01 G_PER_L CHEBI:63004
sodium bromide
 [Merged 2 duplicates: 4.0, 0.01]
19 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
1.81 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
 [Merged 2 duplicates: 1.8, 0.01]
20 H3BO3
H3BO3 61.833 g/mol
1.31 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 1.3, 0.01]
21 KI
I.K 166.002 g/mol
0.01125 G_PER_L CHEBI:8346
potassium iodide
 [Merged 2 duplicates: 0.00125, 0.01]
22 Sodium silicate
2Na.O3Si 122.063 g/mol
0.1 G_PER_L CHEBI:60720
sodium silicate
23 NaF
F.Na 41.988 g/mol
0.06 G_PER_L CHEBI:28741
sodium fluoride
24 KNO3
K.NO3 101.102 g/mol
0.04 G_PER_L CHEBI:63043
potassium nitrate
25 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
0.46 G_PER_L CHEBI:34683
disodium hydrogenphosphate
26 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
27 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
28 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
29 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
30 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
31 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
32 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
33 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
34 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
35 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
36 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
37 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
38 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.5 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
39 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.18 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
40 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
41 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.02 G_PER_L CHEBI:86463
potassium aluminium sulfate
42 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components except iron powder and filter-sterilize. Dispense the medium and iron powder (autoclaved) into sterile culture vessels (typically, 15 - 20 ml medium in 120 ml serum bottles) under a H2-CO2 (80:20, v/v) gas atmosphere and seal with butyl rubber stoppers. Prior to inoculation, reduce the medium with 0.01 volume of 5% Na2S x 9H2O solution (neutralized with H2SO4, autoclaved and stored under a N2 atmosphere). Pressurize the inoculated bottle to 200 kPa H2-CO2 (80:20, v/v).
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.249593Z mediadive-import Imported from MediaDive Source: JCM, ID: J673
2026-03-13T03:09:13.422954Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:17.201440Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003018
2026-03-17T06:30:20.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.856452+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOPYRUS MEDIUM FOR STRAIN 116" to "methanopyrus_medium_for_strain_116" for consistent matching
2026-06-05T07:41:32.127303+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 39 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.478392+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:07.196261+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.