brucella_blood_agar_with_hemin_menadione

CultureMech:003022 bacterial COMPLEX SOLID_AGAR
Original name: BRUCELLA BLOOD AGAR WITH HEMIN MENADIONE

Overview

External ontology
mediadive.medium:J677 (source link unavailable) — BRUCELLA BLOOD AGAR WITH HEMIN MENADIONE
Applications
  • Microbial cultivation
Notes
JCM Medium 677 contains 43.0 g Brucella agar (BD-BBL), 50.0 ml horse blood, 10.0 ml hemin solution, 10.0 ml menadione solution, and 1.0 L distilled water.

Composition

flowchart LR
    CultureMech_003022["brucella_blood_agar_with_hemin_menadione"]
    ing0_Brucella_agar__BD_BBL_["Brucella agar (BD-BBL)"]
    CultureMech_003022 --> ing0_Brucella_agar__BD_BBL_
    ing1_Horse_blood["Horse blood"]
    CultureMech_003022 --> ing1_Horse_blood
    ing2_Distilled_water["Distilled water"]
    CultureMech_003022 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Brucella agar (BD-BBL) 43.0 G_PER_L JCM Medium 677 lists 43.0 g Brucella agar (BD-BBL).
2 Horse blood 50.0 ML_PER_L JCM Medium 677 lists 50.0 ml horse blood.
3 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 JCM Medium 677 lists 1000 ml distilled water.

Preparation

  1. Suspend 43.0 g Brucella agar (BD-BBL) in 1.0 L distilled water.
  2. Add 50.0 ml/L horse blood, 10.0 ml/L hemin solution, and 10.0 ml/L menadione solution.
  3. Autoclave at 121 C for 15 min unless otherwise stated.

Solutions

Hemin solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Hemin 0.5 G_PER_L
1 N NaOH 10.0 ML_PER_L
JCM Medium 677 lists 10.0 ml/L Hemin solution from JCM Medium 469.

Menadione solution

Dose: 10.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Menadione 0.05 G_PER_L
Ethanol 10.0 ML_PER_L
JCM Medium 677 lists 10.0 ml/L Menadione solution from JCM Medium 469.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.265163Z mediadive-import Imported from MediaDive Source: JCM, ID: J677
2026-03-13T06:19:17.228931Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003022
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.888849+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BRUCELLA BLOOD AGAR WITH HEMIN MENADIONE" to "brucella_blood_agar_with_hemin_menadione" for consistent matching
2026-06-05T07:40:51.582333+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_official_simple_score20.py RESOLVED_OFFICIAL_SIMPLE_SCORE20 JCM Medium 677 contains 43.0 g Brucella agar (BD-BBL), 50.0 ml horse blood, 10.0 ml hemin solution, 10.0 ml menadione solution, and 1.0 L distilled water.