alicyclobacillus_ferrooxydans_medium

CultureMech:003030 bacterial COMPLEX LIQUID
Original name: ALICYCLOBACILLUS FERROOXYDANS MEDIUM

Overview

External ontology
mediadive.medium:J685 (source link unavailable) — ALICYCLOBACILLUS FERROOXYDANS MEDIUM
Applications
  • Microbial cultivation
pH
2.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=685

Composition

flowchart LR
    CultureMech_003030["alicyclobacillus_ferrooxydans_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003030 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003030 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_K2HPO4["K2HPO4"]
    CultureMech_003030 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_K2S4O6["K2S4O6"]
    CultureMech_003030 --> ing3_K2S4O6
    CHEBI_86466["CHEBI:86466"]
    ing3_K2S4O6 -.-> CHEBI_86466
    ing4_KCl["KCl"]
    CultureMech_003030 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_003030 --> ing5_Yeast_extract
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003030 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.4 G_PER_L CHEBI:62946
ammonium sulfate
3 K2HPO4
HO4P.2K 174.174 g/mol
0.2 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 K2S4O6
2K.O6S4 302.458 g/mol
0.15 G_PER_L CHEBI:86466
potassium tetrathionate
5 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
6 Yeast extract 0.2 G_PER_L
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
20 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate

Preparation

  1. Adjust pH to 2.5 with H2SO4 and autoclave.
  2. Dissolve in final 100 ml of 0.2 N H2SO4 and filter-sterilize.
  3. To complete the medium, add 70 ml Solution B to 1.0 L Solution A. For solid medium, prepare double-strength Solution A and autoclave. Separately autoclave an equal amount of 1.4% Gerlite (gellan gum) solution. After autoclaving, keep the two solutions at 70C and mix thoroughly. Then, add filter-sterilized Solution B (70 ml/L) and pour into petri dishes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.295138Z mediadive-import Imported from MediaDive Source: JCM, ID: J685
2026-01-28T19:48:22.239361Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:17.300552Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003030
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:30.973891+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALICYCLOBACILLUS FERROOXYDANS MEDIUM" to "alicyclobacillus_ferrooxydans_medium" for consistent matching
2026-06-05T07:40:17.580558+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.068430+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.