Overview
- External ontology
- mediadive.medium:J686 (source link unavailable) — JCM MEDIUM No. 686
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=686
Composition
flowchart LR
CultureMech_003031["jcm_medium_no_686"]
ing0_Sodium_succinate["Sodium succinate"]
CultureMech_003031 --> ing0_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing0_Sodium_succinate -.-> CHEBI_63675
ing1_Bacto_peptone["Bacto peptone"]
CultureMech_003031 --> ing1_Bacto_peptone
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_003031 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003031 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003031 --> ing4_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing4_MnSO4_x_n_H2O -.-> CHEBI_86360
ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_003031 --> ing5_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
ing6_Agar["Agar"]
CultureMech_003031 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
1 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 2 | Bacto peptone | 10 G_PER_L | ||
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.002 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 6 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.002 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.297358Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J686 |
| 2026-03-13T06:19:17.305164Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003031 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:30.978831+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 686" to "jcm_medium_no_686" for consistent matching |
| 2026-06-05T07:41:10.202343+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.318411+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-07T14:52:40.011908+00:00 | codex | CURATED_MEDIUM_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. |