mb_medium_with_formate

Overview

External ontology

mediadive.medium:J688 (source link unavailable) — MB MEDIUM WITH FORMATE

Applications

• Microbial cultivation

Notes

TOGO M708 records JCM Medium 688 as MB Medium With Formate: Solution A contains 920 ml distilled water plus salts, yeast extract, Trypticase peptone, 10 ml trace minerals from M142, and 1 ml selenite-tungstate solution from M431; per liter, add 5 ml each of 5% Na2S x 9H2O and 5% L-cysteine x HCl x H2O, 50 ml of 136 g/L sodium formate stock, and 10 ml trace vitamins from M190.

Composition

flowchart LR
    CultureMech_003033["mb_medium_with_formate"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_003033 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003033 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_003033 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_x_2H2O["CaCl2 x 2H2O"]
    CultureMech_003033 --> ing3_CaCl2_x_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_003033 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_K2HPO4["K2HPO4"]
    CultureMech_003033 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Resazurin["Resazurin"]
    CultureMech_003033 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_MgCl2_x_6H2O["MgCl2 x 6H2O"]
    CultureMech_003033 --> ing7_MgCl2_x_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6H2O -.-> CHEBI_86345
    ing8_KCl["KCl"]
    CultureMech_003033 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_NaHCO3["NaHCO3"]
    CultureMech_003033 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003033 --> ing10_Trypticase_peptone
    ing11_CO2["CO2"]
    CultureMech_003033 --> ing11_CO2
    CHEBI_16526["CHEBI:16526"]
    ing11_CO2 -.-> CHEBI_16526
    ing12_N2["N2"]
    CultureMech_003033 --> ing12_N2
    CHEBI_17997["CHEBI:17997"]
    ing12_N2 -.-> CHEBI_17997

Ingredients (13)

Preparation

1. Mix Solution A components except NaHCO3.
2. Bring to a boil for several seconds and cool under N2-CO2 (80:20).
3. Add NaHCO3, mix thoroughly, and distribute the medium into culture vessels under N2-CO2 (80:20).
4. Seal with butyl rubber stoppers and autoclave.
5. Filter-sterilize the sodium formate solution.
6. Finally, add the sterile 5% Na2S x 9H2O, 5% L-cysteine x HCl x H2O, sodium formate, and trace vitamin solutions per liter.

Solutions

Target organisms

No target organisms are recorded.

Provenance