aerobic_sulfolobales_medium

CultureMech:003037 archaea COMPLEX LIQUID
Original name: AEROBIC SULFOLOBALES MEDIUM

Overview

External ontology
mediadive.medium:J691 (source link unavailable) — AEROBIC SULFOLOBALES MEDIUM
Applications
  • Microbial cultivation
pH
2.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=691

Composition

flowchart LR
    CultureMech_003037["aerobic_sulfolobales_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_003037 --> ing0_Yeast_extract
    ing1_Sulfur["Sulfur"]
    CultureMech_003037 --> ing1_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing1_Sulfur -.-> CHEBI_26833
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003037 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_KH2PO4["KH2PO4"]
    CultureMech_003037 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003037 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003037 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003037 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_003037 --> ing7_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing7_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003037 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003037 --> ing9_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing9_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003037 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_VOSO4_x_n_H2O["VOSO4 x n H2O"]
    CultureMech_003037 --> ing11_VOSO4_x_n_H2O
    CHEBI_87020["CHEBI:87020"]
    ing11_VOSO4_x_n_H2O -.-> CHEBI_87020
    ing12_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003037 --> ing12_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing12_CoSO4_x_7_H2O -.-> CHEBI_91244

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.5 G_PER_L
2 Sulfur
S 32.067 g/mol
1 G_PER_L CHEBI:26833
sulfur atom
3 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.28 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.0018 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0045 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.00022 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
5e-05 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
11 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
3e-05 G_PER_L CHEBI:75213
sodium molybdate dihydrate
12 VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
3e-05 G_PER_L CHEBI:87020
vanadyl sulfate hydrate
13 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
1e-05 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate

Preparation

  1. Autoclave Salt base solution and yeast extract (as a 10% solution) separately and combine after cooling. Steam sulfur for 3 hr on each of 3 successive days.
  2. Adjust pH to 2.5 with 10 N H2SO4.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.308404Z mediadive-import Imported from MediaDive Source: JCM, ID: J691
2026-03-13T06:19:17.334995Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003037
2026-03-17T06:30:11.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.011613+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AEROBIC SULFOLOBALES MEDIUM" to "aerobic_sulfolobales_medium" for consistent matching
2026-06-05T07:40:47.518979+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.673402+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.