spirochaeta_smaeagdinae_medium

CultureMech:003039 bacterial COMPLEX LIQUID
Original name: SPIROCHAETA SMAEAGDINAE MEDIUM

Overview

External ontology
mediadive.medium:J694 (source link unavailable) — SPIROCHAETA SMAEAGDINAE MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=694

Composition

flowchart LR
    CultureMech_003039["spirochaeta_smaeagdinae_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003039 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_NH4Cl["NH4Cl"]
    CultureMech_003039 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_K2HPO4["K2HPO4"]
    CultureMech_003039 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KH2PO4["KH2PO4"]
    CultureMech_003039 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_KCl["KCl"]
    CultureMech_003039 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_003039 --> ing5_Yeast_extract
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003039 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_Resazurin["Resazurin"]
    CultureMech_003039 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003039 --> ing8_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003039 --> ing9_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing10_NaHCO3["NaHCO3"]
    CultureMech_003039 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003039 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003039 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003039 --> ing13_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing14_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003039 --> ing14_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing14_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003039 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003039 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003039 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003039 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003039 --> ing19_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing19_AlK_SO4_2 -.-> CHEBI_86463
    ing20_H3BO3["H3BO3"]
    CultureMech_003039 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003039 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
55.9451 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 54.9451, 1.0]
2 NH4Cl
Cl.H4N 53.492 g/mol
1.0989 G_PER_L CHEBI:31206
ammonium chloride
3 K2HPO4
HO4P.2K 174.174 g/mol
0.362637 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.362637 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 KCl
Cl.K 74.551 g/mol
0.21978 G_PER_L CHEBI:32588
potassium chloride
6 Yeast extract 5.49451 G_PER_L
7 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.549451 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
8 Resazurin
C12H7NO4 229.191 g/mol
0.0010989 G_PER_L CHEBI:8806
Resazurin
9 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
1.92308 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
10 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.061538 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.961538, 0.1]
11 NaHCO3
CHO3.Na 84.006 g/mol
50 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
4 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
15 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
17 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Separately autoclave Solutions A and B under a N2-CO2 (4:1, v/v) gas mixture. After cooling, combine both solutions under the same gas mixture and add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
  2. Check the final pH of the medium to be 7.0.
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.315569Z mediadive-import Imported from MediaDive Source: JCM, ID: J694
2026-03-13T03:09:44.940263Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:17.346598Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003039
2026-03-17T06:30:57.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.024328+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SPIROCHAETA SMAEAGDINAE MEDIUM" to "spirochaeta_smaeagdinae_medium" for consistent matching
2026-06-05T07:41:46.356812+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.646857+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.