gam_broth_with_1_lactose

CultureMech:003042 bacterial COMPLEX LIQUID
Original name: GAM BROTH WITH 1% LACTOSE

Overview

External ontology
mediadive.medium:J697 (source link unavailable) — GAM BROTH WITH 1% LACTOSE
Applications
  • Microbial cultivation
Notes
JCM Medium 697 contains 59.0 g GAM broth (Nissui), 10.0 g lactose, and 1.0 L distilled water.

Composition

flowchart LR
    CultureMech_003042["gam_broth_with_1_lactose"]
    ing0_GAM_broth__Nissui_["GAM broth (Nissui)"]
    CultureMech_003042 --> ing0_GAM_broth__Nissui_
    ing1_Lactose["Lactose"]
    CultureMech_003042 --> ing1_Lactose
    CHEBI_17716["CHEBI:17716"]
    ing1_Lactose -.-> CHEBI_17716
    ing2_Distilled_water["Distilled water"]
    CultureMech_003042 --> ing2_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing2_Distilled_water -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 GAM broth (Nissui) 59.0 G_PER_L JCM Medium 697 lists 59.0 g GAM broth (Nissui).
2 Lactose
C12H22O11 342.297 g/mol
10.0 G_PER_L CHEBI:17716
lactose
JCM Medium 697 lists 10.0 g lactose.
3 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
JCM Medium 697 lists 1000 ml distilled water.

Preparation

  1. Suspend 59.0 g GAM broth (Nissui) and 10.0 g lactose in 1.0 L distilled water.
  2. Autoclave at 121 C for 15 min unless otherwise stated.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-26T17:53:17.328066Z mediadive-import Imported from MediaDive Source: JCM, ID: J697
2026-03-13T06:19:17.376414Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003042
2026-03-17T06:30:00.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.057591+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "GAM BROTH WITH 1% LACTOSE" to "gam_broth_with_1_lactose" for consistent matching
2026-06-05T07:41:05.936334+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_official_simple_score20.py RESOLVED_OFFICIAL_SIMPLE_SCORE20 JCM Medium 697 contains 59.0 g GAM broth (Nissui), 10.0 g lactose, and 1.0 L distilled water.