Overview
- External ontology
- mediadive.medium:J698 (source link unavailable) — ACIDIMICROBIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 1.7
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=698
Composition
flowchart LR
CultureMech_003043["acidimicrobium_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003043 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_003043 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_K2HPO4["K2HPO4"]
CultureMech_003043 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KCl["KCl"]
CultureMech_003043 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003043 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_Yeast_extract["Yeast extract"]
CultureMech_003043 --> ing5_Yeast_extract
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.499002 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.399202 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.199601 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.0998004 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.00998004 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 | Yeast extract | 2.5 G_PER_L |
Preparation
- Adjust pH to 2.0 with H2SO4. After autoclaving, add the following solution (autoclaved):
- Comment: For autotrophic growth on ferrous sulfate, prepare the medium without yeast extract, add 13.9 g/L (final) FeSO4 x 7H2O and adjust pH to 1.7 with H2SO4.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.329338Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J698 |
| 2026-03-13T06:19:17.379880Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003043 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.061463+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDIMICROBIUM MEDIUM" to "acidimicrobium_medium" for consistent matching |
| 2026-06-05T07:40:17.597018+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.075036+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |